The Journal of Microbiology

, Volume 46, Issue 1, pp 75–80

The mutation of a novel Saccharomyces cerevisiae SRL4 gene rescues the Lethality of rad53 and lcd1 mutations by modulating dNTP levels

  • Do-Hee Choi
  • Young-Mi Oh
  • Sung-Hun Kwon
  • Sung-Ho Bae
Article

DOI: 10.1007/s12275-008-0013-6

Cite this article as:
Choi, D., Oh, Y., Kwon, S. et al. J Microbiol. (2008) 46: 75. doi:10.1007/s12275-008-0013-6

Abstract

The SRL4 (YPL033C) gene was initially identified by the screening of Saccharomyces cerevisiae genes that play a role in DNA metabolism and/or genome stability using the SOS system of Escherichia coli. In this study, we found that the srl4Δ mutant cells were resistant to the chemicals that inhibit nucleotide metabolism and evidenced higher dNTP levels than were observed in the wild-type cells in the presence of hydroxyurea. The mutant cells also showed a significantly faster growth rate and higher dNTP levels at low temperature (16°C) than were observed in the wild-type cells, whereas we detected no differences in the growth rate at 30°C. Furthermore, srl4Δ was shown to suppress the lethality of mutations of the essential S phase checkpoint genes, RAD53 and LCD1. These results indicate that SRL4 may be involved in the regulation of dNTP production by its function as a negative regulator of ribonucleotide reductase.

Keywords

SRL4nucleotide metabolismribonucleotide reductaseSML1checkpointSaccharomyces cerevisiae

Copyright information

© The Microbiological Society of Korea 2008

Authors and Affiliations

  • Do-Hee Choi
    • 1
  • Young-Mi Oh
    • 1
  • Sung-Hun Kwon
    • 1
  • Sung-Ho Bae
    • 1
  1. 1.Department of Biological Sciences, College of Natural ScienceInha UniversityIncheonRepublic of Korea