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Ferroxidase-like activity of Au nanorod/Pt nanodot structures and implications for cellular oxidative stress

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Abstract

Platinum nanoparticles (NPs) are reported to mimic various antioxidant enzymes and thus may produce a positive biological effect by reducing reactive oxygen species (ROS) levels. In this manuscript, we report Pt NPs as an enzyme mimic of ferroxidase by depositing platinum nanodots on gold nanorods (Au@Pt NDRs). Au@Pt NDRs show pH-dependent ferroxidase-like activity and have higher activity at neutral pH values. Cytotoxicity results with human cell lines (lung adenocarcinoma A549 and normal bronchial epithelial cell line HBE) show that Au@Pt NDRs are taken up into cells via endocytosis and translocate into the endosome/lysosome. Au@Pt NDRs have good biocompatibility at NDR particle concentrations lower than 0.15 nΜ. However, in the presence of H2O2, lysosomelocated NDRs exhibit peroxidase-like activity and therefore increase cytotoxicity. In the presence of Fe2+, the ferroxidase-like activity of the NDRs protects cells from oxidative stress by consuming H2O2. Thorough consideration should be given to this behavior when employing Au@Pt NDRs in biological systems.

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Authors and Affiliations

  1. CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, 100190, China

    Jianbo Liu, Zhijian Hu, Tao Wen, Wenqi Liu & Xiaochun Wu

  2. College of Opto-electronic Engineering, Zaozhuang University, Zaozhuang, 277100, China

    Jianbo Liu

  3. CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190, China

    Xiumei Jiang, Liming Wang & Chunying Chen

  4. Division of Analytical Chemistry, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD, 20740, USA

    Wenqi Liu & Junjie Yin

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  1. Jianbo Liu
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  5. Tao Wen
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Correspondence to Junjie Yin, Chunying Chen or Xiaochun Wu.

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Liu, J., Jiang, X., Wang, L. et al. Ferroxidase-like activity of Au nanorod/Pt nanodot structures and implications for cellular oxidative stress. Nano Res. 8, 4024–4037 (2015). https://doi.org/10.1007/s12274-015-0904-x

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  • DOI: https://doi.org/10.1007/s12274-015-0904-x

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