Nano Research

, 4:921

Highly sensitive protein sensor based on thermally-reduced graphene oxide field-effect transistor

Research Article

DOI: 10.1007/s12274-011-0148-3

Cite this article as:
Mao, S., Yu, K., Lu, G. et al. Nano Res. (2011) 4: 921. doi:10.1007/s12274-011-0148-3


We report the fabrication of a highly sensitive field-effect transistor (FET) biosensor using thermally-reduced graphene oxide (TRGO) sheets functionalized with gold nanoparticle (NP)-antibody conjugates. Probe antibody was labeled on the surface of TRGO sheets through Au NPs and electrical detection of protein binding (Immunoglobulin G/IgG and anti-Immunoglobulin G/anti-IgG) was accomplished by FET and direct current (dc) measurements. The protein binding events induced significant changes in the resistance of the TRGO sheet, which is referred to as the sensor response. The dependence of the sensor response on the TRGO base resistance in the sensor and the antibody areal density on the TRGO sheet was systematically studied, from which a correlation of the sensor response with sensor parameters was found: the sensor response was more significant with larger TRGO base resistance and higher antibody areal density. The detection limit of the novel biosensor was around the 0.2 ng/mL level, which is among the best of reported carbon nanomaterial-based protein sensors and can be further optimized by tuning the sensor structure.


Biosensorthermally-reduced graphene oxidefield-effect transistorprotein detection

Supplementary material

12274_2011_148_MOESM1_ESM.pdf (279 kb)
Supplementary material, approximately 278 KB.

Copyright information

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  1. 1.Department of Mechanical EngineeringUniversity of Wisconsin-MilwaukeeMilwaukeeUSA