The osmotic stress response of split influenza vaccine particles in an acidic environment
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Oral influenza vaccine provides an efficient means of preventing seasonal and pandemic disease. In this work, the stability of envelope-type split influenza vaccine particles in acidic environments has been investigated. Owing to the fact that hyper-osmotic stress can significantly affect lipid assembly of vaccine, osmotic stress-induced morphological change of split vaccine particles, in conjunction with structural change of antigenic proteins, was investigated by the use of stopped-flow light scattering (SFLS), intrinsic fluorescence, transmission electron microscopy (TEM), and hemagglutination assay. Split vaccine particles were found to exhibit a step-wise morphological change in response to osmotic stress due to double-layered wall structure. The presence of hyper-osmotic stress in acidic medium (0.3 osmolarity, pH 2.0) induced a significant level of membrane perturbation as measured by SFLS and TEM, imposing more damage to antigenic proteins on vaccine envelope than can be caused by pH-induced conformational change at acidic iso-osmotic condition. Further supports were provided by the intrinsic fluorescence and hemagglutinin activity measurements. Thus, hyper-osmotic stress becomes an important factor for determining stability of split vaccine particles in acidic medium. These results are useful in better understanding the destabilizing mechanism of split influenza vaccine particles in gastric environment and in designing oral influenza vaccine formulations.
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- The osmotic stress response of split influenza vaccine particles in an acidic environment
Archives of Pharmacal Research
Volume 37, Issue 12 , pp 1607-1616
- Cover Date
- Print ISSN
- Online ISSN
- Pharmaceutical Society of Korea
- Additional Links
- Influenza vaccine
- Split vaccine particle
- Whole inactivated virus vaccine particle
- Osmotic stress
- Stopped-flow light scattering
- Industry Sectors
- Author Affiliations
- 1. Nanotechnology Accelerator and Department of Chemical and Materials Engineering, National Institute for Nanotechnology, University of Alberta, Edmonton, AB, T6G 2M9, Canada
- 2. Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, 430-757, Korea
- 3. Center for Inflammation, Immunity and Infection and Department of Biology, Georgia State University, Atlanta, GA, 30303, USA