Archives of Pharmacal Research

, Volume 37, Issue 7, pp 899–906

Metabonomics study on the hot syndrome of traditional Chinese medicine by rapid resolution liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry

  • Yang Wang
  • Li Ma
  • Yi Sun
  • Liman Yang
  • Hao Yue
  • Shuying Liu
Research Article

DOI: 10.1007/s12272-013-0250-z

Cite this article as:
Wang, Y., Ma, L., Sun, Y. et al. Arch. Pharm. Res. (2014) 37: 899. doi:10.1007/s12272-013-0250-z

Abstract

The hot syndrome refers to any feverish conditions during a pathological development, a sub-health phenomenon, and is a potential risk for human health. The metabonomics study on the hot syndrome may provide insight into understanding of its pathology and play a role in the prevention and treatment of its related diseases. In this paper, the rats were dosed with the hot syndrome prescription, ginseng and water. The corresponding urine samples were identified by rapid resolution liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry. More than 1,000 metabolic compounds from different urine samples could be further differentiated by principal component analysis. As a result, the rat body temperature and weight were recognized as the hot syndrome related factors. Some specific metabolites have been discovered as a pattern of the potential biomarkers for the hot syndrome. The results showed that ginseng cannot cause the hot syndrome in a reasonable dose, but the hot syndrome prescription can. It is suggested that ginseng cannot be used only as a tradition Chinese medicine but also as a nutrient. The work showed metabonomics method is a valuable tool in studying mechanism of the hot syndrome.

Keywords

The hot syndromeMetabonomicsMass spectrometryPrincipal component analysis

Supplementary material

12272_2013_250_MOESM1_ESM.tif (389 kb)
Loading plot of PCA for common components in positive ion mode (A) and in negative ion mode (B) (TIFF 389 kb)
12272_2013_250_MOESM2_ESM.tif (59 kb)
(A) extract ion chromatography and (B) mass spectrum of the ion at m/z295.2273 (TIFF 59 kb)
12272_2013_250_MOESM3_ESM.tif (1 mb)
Scores plot of PCA of control group ■, the hot syndrome group ▲ and ginseng administrated group ● in positive ion mode (A) and in negative ion mode (B) (TIFF 1035 kb)
12272_2013_250_MOESM4_ESM.tif (277 kb)
Summary of pathway analysis with MetPA. (1) Amino sugar and nucleotide sugar metabolism; (2) Arginine and proline metabolism; (3) Primary bile acid biosynthesis; (4) Lysine biosynthesis; (5) alpha-Linolenic acid metabolism; (6) Pyrimidine metabolism; (7) Lysine degradation; (8) Phenylalanine metabolism; (9) Folate biosynthesis (TIFF 276 kb)
12272_2013_250_MOESM5_ESM.doc (42 kb)
Supplementary material 5 (DOC 42 kb)
12272_2013_250_MOESM6_ESM.doc (35 kb)
Supplementary material 6 (DOC 35 kb)

Copyright information

© The Pharmaceutical Society of Korea 2013

Authors and Affiliations

  • Yang Wang
    • 1
  • Li Ma
    • 2
    • 3
  • Yi Sun
    • 1
  • Liman Yang
    • 1
  • Hao Yue
    • 1
  • Shuying Liu
    • 1
  1. 1.Jilin Ginseng AcademyChangchun University of Chinese MedicineChangchunChina
  2. 2.Chongqing Key Laboratory for Diseases Proteomics, Institute of Burn Research, Southwest HospitalThird Military Medical UniversityChongqingChina
  3. 3.School of Environmental Air Security and Pollution Control EngineeringJinan UniversityGuangzhouChina