Archives of Pharmacal Research

, Volume 36, Issue 7, pp 912–917

Antioxidant effect of astragalin isolated from the leaves of Morus alba L. against free radical-induced oxidative hemolysis of human red blood cells


  • Jiwon Choi
    • Department of Radiological SciencesJeonju University
    • Department of Physiology, School of MedicineChonbuk National University
  • Hyun Ju Kang
    • Department of Healthcare & ScienceJeonju University
  • Sung Zoo Kim
    • Department of Physiology, School of MedicineChonbuk National University
  • Tae Oh Kwon
    • College of Life Science and Natural ResourcesWonkwang University
  • Seung-Il Jeong
    • Jeonju Biomaterials Institute
    • Department of Healthcare & ScienceJeonju University
Research Article

DOI: 10.1007/s12272-013-0090-x

Cite this article as:
Choi, J., Kang, H.J., Kim, S.Z. et al. Arch. Pharm. Res. (2013) 36: 912. doi:10.1007/s12272-013-0090-x


We evaluated the antioxidant properties of mulberry leaves extract (MLE) and flavonoids isolated from MLE. MLE was prepared by extraction with methanol. Flavonoids were analyzed by high-performance liquid chromatography. Oxidative hemolysis of normal human red blood cells (RBCs) was induced by the aqueous peroxyl radical [2,2′-Azobis (2-amidinopropane) dihydrochloride, AAPH]. MLE contained three flavonoids in the order quercetin (QC) > kaempferol (KF) > astragalin (AG). Oxidative hemolysis of RBCs induced by AAPH was suppressed by MLE, AG, KF, and QC in a time- and dose-dependent manner. MLE and these three flavonoids prevented the depletion of cystosolic antioxidant glutathione (GSH) in RBCs. AG had the greatest protective effect against AAPH-induced oxidative hemolysis and GSH depletion in RBCs.


Antioxidant activity Astragalin Flavonoid Mulberry leaves Oxidative damage Red blood cells

Supplementary material

12272_2013_90_MOESM1_ESM.pdf (40 kb)
Supplementary material 1 (PDF 40 kb)

Copyright information

© The Pharmaceutical Society of Korea 2013