Archives of Pharmacal Research

, Volume 36, Issue 6, pp 723–730

Antibacterial substances from Albizia myriophylla wood against cariogenic Streptococcus mutans

  • Nantiya Joycharat
  • Sonesay Thammavong
  • Surasak Limsuwan
  • Sirilux Homlaead
  • Supayang Piyawan Voravuthikunchai
  • Boon-ek Yingyongnarongkul
  • Sukanya Dej-adisai
  • Sanan Subhadhirasakul
Research Article

DOI: 10.1007/s12272-013-0085-7

Cite this article as:
Joycharat, N., Thammavong, S., Limsuwan, S. et al. Arch. Pharm. Res. (2013) 36: 723. doi:10.1007/s12272-013-0085-7

Abstract

Albizia myriophylla has been used for long by Thai traditional healers as an important ingredient herb in Thai herbal formulas for caries. In this study, three flavonoids lupinifolin (6), 8-methoxy-7,3′,4′-trihydroxyflavone (7), and 7,8,3′,4′-tetrahydroxyflavone (8), a triterpenoid lupeol (3) as well as four sterols β-sitosterone (1), stigmasta-5,22-dien-3-one (2), β-sitosterol (4), and stigmasterol (5) were isolated from A. myriophylla wood. The antibacterial activity of these compounds against Streptococcus mutans ATCC 25175 was performed using broth microdilution method. All compounds exhibited antibacterial activity against S. mutans with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) ranging from 1–256 and 2–256 μg/ml, respectively. Among the isolated compounds, lupinifolin (6) was found to be the most potent with MIC and MBC of 1 and 2 μg/ml, respectively. Lupinifolin (6) also showed a strong activity against ten clinical isolates of S. mutans with MIC and MBC ranging from 0.25–2 and 0.5–8 μg/ml, respectively. These results reported the bioactive ingredients of A. myriophylla which support its ethnomedical claims as well. Lupinifolin (6) may have a potential to be a natural anticariogenic agent.

Keywords

Albizia myriophyllaLeguminosaeLupinifolinFlavonoidStreptococcus mutansMedicinal plant

Copyright information

© The Pharmaceutical Society of Korea 2013

Authors and Affiliations

  • Nantiya Joycharat
    • 3
    • 1
  • Sonesay Thammavong
    • 1
  • Surasak Limsuwan
    • 1
    • 3
  • Sirilux Homlaead
    • 2
  • Supayang Piyawan Voravuthikunchai
    • 2
    • 3
  • Boon-ek Yingyongnarongkul
    • 4
  • Sukanya Dej-adisai
    • 5
  • Sanan Subhadhirasakul
    • 1
    • 5
  1. 1.Faculty of Traditional Thai Medicine, Prince of Songkla UniversitySongkhlaThailand
  2. 2.Department of MicrobiologyFaculty of Science, Prince of Songkla UniversitySongkhlaThailand
  3. 3.Natural Products Research CenterFaculty of Science, Prince of Songkla UniversitySongkhlaThailand
  4. 4.Department of ChemistryFaculty of Science, Ramkhamhaeng UniversityBangkokThailand
  5. 5.Faculty of Pharmaceutical SciencesPrince of Songkla UniversitySongkhlaThailand