Research Articles Drug Development

Archives of Pharmacal Research

, Volume 34, Issue 10, pp 1663-1678

First online:

Moxifloxacin-gelrite In Situ ophthalmic gelling system against photodynamic therapy for treatment of bacterial corneal inflammation

  • Hanan M. El-LaithyAffiliated withDepartment of Pharmaceutics and Industrial Pharmacy, Pharmacy College, Cairo University
  • , Demiana I. NesseemAffiliated withDepartment of Pharmaceutics, National Organization for Drug Control and ResearchDepartment of Pharmaceutics, National Organization for Drug Control and Research (NODCAR) Email author 
  • , Amira A. El-AdlyAffiliated withLaser Microbiological Lab., National Institute of Laser Enhanced Science (NILES), Cairo University
  • , Meriana ShoukryAffiliated withDepartment of Pharmaceutics, National Organization for Drug Control and Research

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In this study, six in situ gelling formulations based on Gelrite were prepared and evaluated for the retained ophthalmic delivery of Moxifloxacin (Mox). The effectiveness of the best developed formula G5 was compared with photodynamic therapy (PDT), the recent expanding approach for the treatment of ophthalmologic disorders after the assessment of optimum photodynamic inactivation parameters that permit efficient pathogens eradication. It was found that, Staphylococcus aureus (S. aureus) (Gram-positive) was more susceptible to effective lethal photosensitization that reaches 93.5% reduction in viable count than Escherichia coli (E. coli) (Gramnegative) of 76.1% using 3 mg/mL Hematoporphyrin (HP), illuminated by 630 nm Light Emitting Diode (LED) at 9 J/cm2 and incubated for 15 min. Following topical instillation of G5 to rabbits corneas, higher amount of Mox was retained in the aqueous humor up to 24 h with significant 6-fold increase in the Cmax and AUC(0-∞) compared to vigamox® commercial eye drops. After post corneal infection with S. aureus, both approaches were effectively treating the infection without causing ocular irritation or collateral damage to corneal tissue where G5 showed remarkable improvement after four days compared to seven days of PDT treatment.

Key words

Moxifloxacin Ocular delivery In situ gelling systems Gelrite® Photodynamic therapy