Archives of Pharmacal Research

, Volume 33, Issue 11, pp 1843–1850

Anti-inflammatory effect of visnagin in lipopolysaccharide-stimulated BV-2 microglial cells

  • Jin-Koo Lee
  • Jun-Sub Jung
  • Sang-Hee Park
  • Soo-Hyun Park
  • Yun-Beom Sim
  • Seon-Mi Kim
  • Tal-Soo Ha
  • Hong-Won Suh
Article

DOI: 10.1007/s12272-010-1117-1

Cite this article as:
Lee, JK., Jung, JS., Park, SH. et al. Arch. Pharm. Res. (2010) 33: 1843. doi:10.1007/s12272-010-1117-1

Abstract

Visnagin, which is found in Ammi visnaga, has biological activity as a vasodilator and reduces blood pressure by inhibiting calcium influx into the cell. The present study demonstrates the anti-inflammatory effect of visnagin on lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. When cells were treated with visnagin prior to LPS stimulation, production of nitric oxide and expression of iNOS were attenuated in a dose-dependent manner. Visnagin also caused a significant decrease of mRNA expression and release of TNF-α, IL-1β and IFNγ. In addition, visnagin reduced LPS-induced IL-6 and MCP-1 mRNA level. We further found that visnagin dose-dependently inhibited LPS-induced AP-1 and NF-κB luciferase activities. Taken together, our results for the first time suggest that the anti-inflammatory effect of visnagin might result from the inhibition of transcription factors, such as AP-1 and NF-κB.

Key words

Anti-inflammationMicroglial cellsVisnaginInducible nitric oxide synthaseAP-1NF-κB

Copyright information

© The Pharmaceutical Society of Korea and Springer Netherlands 2010

Authors and Affiliations

  • Jin-Koo Lee
    • 1
  • Jun-Sub Jung
    • 1
  • Sang-Hee Park
    • 1
  • Soo-Hyun Park
    • 1
  • Yun-Beom Sim
    • 1
    • 2
  • Seon-Mi Kim
    • 1
    • 2
  • Tal-Soo Ha
    • 3
  • Hong-Won Suh
    • 1
    • 2
  1. 1.Institute of Natural MedicineHallym UniversityChuncheonKorea
  2. 2.Department of Pharmacology, College of MedicineHallym UniversityChuncheonKorea
  3. 3.Department of Molecular Biology, College of Natural ScienceDaegu UniversityGyeongbukKorea