Archives of Pharmacal Research

, Volume 33, Issue 9, pp 1307–1315

Inhibition of DNA topoisomerases I and II and cytotoxicity of compounds from Ulmus davidiana var. japonica

Authors

  • Ming Shan Zheng
    • College of PharmacyYeungnam University
    • Yanbian University
  • Yeun-Kyung Lee
    • College of PharmacyYeungnam University
  • Ying Li
    • College of PharmacyYeungnam University
  • Kyoung Hwangbo
    • College of PharmacyYeungnam University
  • Chong-Soon Lee
    • Department of Biochemistry, College of ScienceYeungnam University
  • Jae-Ryong Kim
    • Department of Biochemistry and Molecular Biology, Aging-associated Vascular Disease Research Center, College of MedicineYeungnam University
  • Sunny Kyung-Seon Lee
    • Department of Pathology and Laboratory MedicineUniversity of California Davis
  • Hyun-Wook Chang
    • College of PharmacyYeungnam University
    • College of PharmacyYeungnam University
Research Articles Drug Discovery and Development

DOI: 10.1007/s12272-010-0903-0

Cite this article as:
Zheng, M.S., Lee, Y., Li, Y. et al. Arch. Pharm. Res. (2010) 33: 1307. doi:10.1007/s12272-010-0903-0

Abstract

Twenty five compounds including ten triterpenes (1–3, 5–11), six flavonoids (12–15, 24, 25), five lignans (17, 18, 21–23), two butenyl clohexnone glycosides (19–20), one fructofuranoside (16) and one fatty acid (4) were isolated from the roots of Ulmus davidiana var. japonica. The structures of those compounds were identified by comparing their physicochemical and spectral data with those of published in literatures. All the compounds were evaluated for DNA topoisomerase inhibitory activities and cytotoxicities. Among the purified compounds, 4 and 19 showed more potent inhibitory acitivities (IC50: 39 and 19 μM, respectively) than camptothecin, as the positive control (IC50: 46 μM) against topoisomerase I. Compounds, 4, 10, 12, 19, 24 and 25 showed strong inhibitory activities toward DNA topoisomerase II (IC50: 0.1, 0.52, 0.47, 0.42, 0.17 μM and 17 nM, respectively), which were more potent than that of etoposide as positive control (IC50: 20 μM). In A549 cell line, 5 and 6 showed cytotoxicities (IC50: 4 μM and 3 μM, respectively, with IC50 of camptothecin as positive control: 10.3 μM). In the HepG2 cell line, 3, 5 and 7 showed cytotoxicity (IC50: 4, 3 and 4 μM, respectively, with IC50 of camptothecin: 0.3 μM). Compounds 6, 12 and 23 showed cytotoxicities in the HT-29 cell line (IC50: 19, 19 and 15 μM, respectively, with IC50 of camptothecin: 2 μM).

Key words

Ulmus davidiana var. japonicaTopoisomeraseCytotoxicity

Copyright information

© The Pharmaceutical Society of Korea and Springer Netherlands 2010