Archives of Pharmacal Research

, Volume 33, Issue 4, pp 625–632

Formononetin prevents ovariectomy-induced bone loss in rats

  • Hyekyung Ha
  • Ho Young Lee
  • Je-Hyun Lee
  • Dayoung Jung
  • Jiyoon Choi
  • Kye-Yong Song
  • Hee Jin Jung
  • Jae Sue Choi
  • Soo-Ik Chang
  • Chungsook Kim
Research Articles Drug Actions

DOI: 10.1007/s12272-010-0418-8

Cite this article as:
Ha, H., Lee, H.Y., Lee, JH. et al. Arch. Pharm. Res. (2010) 33: 625. doi:10.1007/s12272-010-0418-8

Abstract

The major risk factor of postmenopausal osteoporosis is estrogen deficiency. Hormone replacement therapy is efficacious against osteoporosis, but it induces several significant adverse effects. In this study, therefore, we compared therapeutic potencies of three phytoestrogens: genistein, daidzein, and formononetin. Our result showed that in Saos-2 cells, formononetin and genistein (5 × 10−7 M) treatment increased alkaline phosphatase activity by 33.0 ± 5.8% and 21.1 ± 4.0%. Genistein inhibited osteoclast formation in a dose-dependent manner. In OVX rats, formononetin-treated groups given 1 and 10 mg/kg/day displayed increased trabecular bone areas (TBAs) within the tibia. Genistein- and daidzein-treated groups also displayed increased tibial TBAs. TBAs of the lumbar vertebrae were higher in all treated groups than in the control group. In conclusion, formononetin as well as other isoflavones, such as daidzein and genistein, inhibited bone loss caused by estrogen-deficiency.

Key words

Osteoporosis Isoflavones Osteoblast Osteoclast Ovariectomized rat 

Copyright information

© The Pharmaceutical Society of Korea and Springer Netherlands 2010

Authors and Affiliations

  • Hyekyung Ha
    • 1
  • Ho Young Lee
    • 1
  • Je-Hyun Lee
    • 2
  • Dayoung Jung
    • 1
  • Jiyoon Choi
    • 1
  • Kye-Yong Song
    • 3
  • Hee Jin Jung
    • 4
  • Jae Sue Choi
    • 4
  • Soo-Ik Chang
    • 5
  • Chungsook Kim
    • 6
  1. 1.Herbal Medicine EBM Research CenterKorea Institute of Oriental MedicineDaejeonKorea
  2. 2.College of Oriental MedicineDongguk UniversityKyeongjuKorea
  3. 3.College of MedicineChung-Ang UniversitySeoulKorea
  4. 4.Division of Food Science and BiotechnologyPukyong National UniversityBusanKorea
  5. 5.School of Life SciencesChungbuk National UniversityChongjuKorea
  6. 6.Department of Food and NutritionAndong National UniversityAndongKorea

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