Archives of Pharmacal Research

, Volume 32, Issue 6, pp 857–862

Bile acid derivatives from a sponge-associated bacterium Psychrobacter sp.

  • Huayue Li
  • Pramod B. Shinde
  • Hye Ja Lee
  • Eun Sook Yoo
  • Chong-O. Lee
  • Jongki Hong
  • Sang Ho Choi
  • Jee H. Jung
Research Articles Drug Discovery and Development

DOI: 10.1007/s12272-009-1607-1

Cite this article as:
Li, H., Shinde, P.B., Lee, H.J. et al. Arch. Pharm. Res. (2009) 32: 857. doi:10.1007/s12272-009-1607-1

Abstract

In our search for bioactive metabolites from a marine sponge-associated bacterium Psychrobacter sp., a new bile acid derivative (1), which was assumed to be an artifact, were isolated along with six known (27) compounds by bioactivity-guided fractionation. Elucidation of the structure of the new compound was done using a combination of NMR (1H, 13C, HMBC, HSQC, and COSY) and MS spectroscopy. Compound 1 exhibited moderate suppressive effects on both NO and IL-6 production at a concentration of 200 μM (87.3 μg/mL) without significant cytotoxicity against cells. Compounds 25 and 7 showed selective inhibitory activity against several human pathogenic bacterial strains at the low concentration of 30 μg/well. In a cytotoxicity evaluation, only compound 7 showed mild cytotoxicity against five human solid tumor cell lines (A-549, SK-OV-3, SK-MEL-2, XF-498, and HCT-15) with ED50 values in the range of 11–14 μg/mL.

Key words

Sponge-associatedPsychrobacter sp.Bile acidAnti-inflammatoryAntibacterialCytotoxicity

Copyright information

© The Pharmaceutical Society of Korea 2009

Authors and Affiliations

  • Huayue Li
    • 1
  • Pramod B. Shinde
    • 1
  • Hye Ja Lee
    • 2
  • Eun Sook Yoo
    • 2
  • Chong-O. Lee
    • 3
  • Jongki Hong
    • 4
  • Sang Ho Choi
    • 5
  • Jee H. Jung
    • 1
  1. 1.College of PharmacyPusan National UniversityBusanKorea
  2. 2.College of MedicineCheju National UniversityJejuKorea
  3. 3.Pharmaceutical Screening CenterKorea Research Institute of Chemical TechnologyDaejonKorea
  4. 4.College of PharmacyKyung Hee UniversitySeoulKorea
  5. 5.Department of Agricultural BiotechnologySeoul National UniversitySeoulKorea