Archives of Pharmacal Research

, 31:1281

Antiproliferative effects of quercetin through cell cycle arrest and apoptosis in human breast cancer MDA-MB-453 cells

Research Article Drug Efficacy and Safety

DOI: 10.1007/s12272-001-2107-0

Cite this article as:
Choi, E.J., Bae, S.M. & Ahn, W.S. Arch. Pharm. Res. (2008) 31: 1281. doi:10.1007/s12272-001-2107-0
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Abstract

To explore the anticancer effects of the flavonoid quercetin on human breast cancer MDA-MB-453 cells via cell cycle regulation and the induction of apoptosis, the antiproliferative effect of quercetin was first examined by MTT assay. When MDA-MB-453 cells were treated with quercetin for various periods of time (3–24 hrs) and at various doses (1–100 μM), cell growth decreased significantly in a time-and dose-dependent manner. To elucidate the mechanism underlying the antiproliferative effect of quercetin, cell cycle progression and the induction of apoptosis in MDA-MB-453 cells exposed to 100 μM quercetin for 24 hrs were investigated. Quercetin caused a remarkable increase in the number of sub-G1 phase cells, and an Annexin-V assay revealed that exposure to quercetin affected apoptosis. Moreover, treatment with quercetin increased Bax expression but decreased Bcl-2 expression. Cleaved caspase-3 and PARP expression was also increased by quercetin. Thus, quercetin has probable anticancer activity. Our results suggest the existence of multiple pathways for the induction of cell cycle arrest and apoptosis by quercetin.

Key words

ApoptosisCell cycle arrestHuman breast cancer MDA-MB-453 cellsQuercetin

Copyright information

© The Pharmaceutical Society of Korea 2008

Authors and Affiliations

  • Eun Jeong Choi
    • 2
  • Su Mi Bae
    • 2
  • Woong Shick Ahn
    • 1
    • 2
  1. 1.Department of Obstetrics and Gynecology, College of MedicineThe Catholic University of KoreaSeoulKorea
  2. 2.Cancer Research InstituteThe Catholic University of KoreaSeoulKorea