Articles Drug Efficacy and Safety

Archives of Pharmacal Research

, 31:659

First online:

Effects of tanshinone IIA on the hepatotoxicity and gene expression involved in alcoholic liver disease

  • Hu-Quan YinAffiliated withCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
  • , Youn-Su KimAffiliated withCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
  • , You-Jin ChoiAffiliated withCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
  • , Youn-Chul KimAffiliated withCollege of Pharmacy, Wonkwang UniversityCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
  • , Dong-Hwan SohnAffiliated withCollege of Pharmacy, Wonkwang UniversityCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
  • , Shi-Yong RyuAffiliated withKorea Research Institute of Chemical TechnologyCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
  • , Byung-Hoon LeeAffiliated withCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University Email author 

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Abstract

Tanshinone IIA is one of the most abundant constituents of the root of Salvia miltiorrhiza BUNGE which exerts antioxidant and anti-inflammatory actions in many experimental disease models. In the present study, we demonstrated that the standardized fraction of S. miltiorrhiza (Sm-SF) was able to protect RAW 264.7 cells from ethanol-and lipopolysaccharide (LPS)-induced production of superoxide radical, activation of NADPH oxidase and subsequently death of the cells. Among four main components of Sm-SF, tanshinone IIA was the most potent in protecting cells from LPS-and ethanol-induced cytotoxicity. LPS or ethanol induced the expression of CD14, iNOS, and SCD1 and decreased RXR-α, which was completely reversed by tanshinone IIA. In H4IIEC3 cells, 10 μM tanshinone IIA effectively blocked ethanol-induced fat accumulation as evidenced by Nile Red binding assay. These results indicate that tanshinone IIA may have potential to inhibit alcoholic liver disease by reducing LPS-and ethanol-induced Kupffer cell sensitization, inhibiting synthesis of reactive oxygen/nitrogen species, inhibiting fatty acid synthesis and stimulating fatty acid oxidation.

Key words

Salvia miltiorrhiza Tanshinone IIA Alcoholic liver disease Oxidative stress Lipid metabolism