Archives of Pharmacal Research

, Volume 31, Issue 5, pp 617–621

5-Methoxy-8-(2-hydroxy-3-buthoxy-3-methylbutyloxy)-psoralen isolated from Angelica dahurica inhibits cyclooxygenase-2 and 5-Lipoxygenase in mouse bone marrow-derived mast cells

Articles Drug Efficacy and Safety

DOI: 10.1007/s12272-001-1202-9

Cite this article as:
Hua, J.M., Moon, T.C., Hong, T.G. et al. Arch. Pharm. Res. (2008) 31: 617. doi:10.1007/s12272-001-1202-9

Abstract

5-Methoxy-8-(2-hydroxy-3-buthoxy-3-methylbutyloxy)-psoralen (MP) is a medicinal herbal product isolated from Angelica dahurica that inhibits the cyclooxygenase-2 (COX-2)-dependent phase of prostaglandin D2 (PGD2) generation in bone marrow-derived mast cells (IC50, 23.5 μM). Western blotting with specific anti-COX-2 antibodies showed that the decrease in PGD2 production was accompanied by a decrease in COX-2 protein levels. In addition, this compound consistently inhibited the production of leukotriene C4 in a dose dependent manner, (IC50, 2.5 μM). These results demonstrate that MP inhibits both cyclooxygenase-2 and 5-lipoxygenase activity. Furthermore, this compound also inhibited the degranulation reaction (IC50, 4.1 μM). Therefore, this compound might provide a basis for novel anti-inflammatory drug development.

Key words

Angelica dahuricaCyclooxygenase-25-LipoxygenaseBone marrow-derived mast cellsInflammation

Copyright information

© The Pharmaceutical Society of Korea 2008

Authors and Affiliations

  • Jin Mei Hua
    • 1
  • Tae Chul Moon
    • 2
    • 1
  • Tae Gyun Hong
    • 1
  • Kyong Min Park
    • 1
  • Jong Keun Son
    • 1
  • Hyeun Wook Chang
    • 1
  1. 1.College of PharmacyYeungnam UniversityGyongsanKorea
  2. 2.Pulmonary Research Group, Department of MedicineUniversity of AlbertaEdmontonCanada