Articles Drug Efficacy and Safety

Archives of Pharmacal Research

, Volume 31, Issue 2, pp 216-224

First online:

Effects of ginkgo biloba on in vitro osteoblast cells and ovariectomized rat osteoclast cells

  • Seung Min OhAffiliated withCollege of Pharmacy, Sungkyunkwan University
  • , Ha Ryong KimAffiliated withCollege of Pharmacy, Sungkyunkwan University
  • , Kyu Hyuck ChungAffiliated withCollege of Pharmacy, Sungkyunkwan University Email author 

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Ginkgo biloba extract (GBE) has a selective estrogen receptor modulator (SERM)-like biphasic effect on estrogen, and could be a potential alternative to hormone replacement therapy (HRT). Here, we investigated whether GBE can ameliorate estrogen-depleted osteoporosis in in vitro osteoblast cells and in estrogen-deprived ovariectomized (OVX) rats, a classical animal model for postmenopausal osteoporosis. GBE (50–150 μg/mL) significantly increased ALP (Alkaline phosphatase) activity of osteoblast cells, indicating that GBE promotes osteoblast mineralization. OVX rats exposed to GBE (100 and 200 mg/kg/day, oral treatment), raloxifene (3 mg/kg/day, oral treatment) or estradiol (E2, 10 μg/kg/day, subcutaneous injection) decreased osteoclast resorptive activity compared with OVX rats. GBE and raloxifene did not increase uterine weight compared with OVX rats, while E2 and Sham control did, suggesting that GBE has no uterotrophic activity, which is a disadvantage of estrogen therapy. In OVX rats, GBE did not restore severe bone density loss induced by OVX, indicating that GBE may be insufficient as therapeutic material for severe osteoporosis. However, despite its no effects on bone density loss in OVX rats, GBE did stimulate osteoblast differentiation and antiosteoclastic activity in vitro. Therefore, GBE may have preventive potential on osteoporosis as do other phytoestrogens.

Key words

Ginkgo biloba extracts Osteoporosis Ovariectomized rat Osteoclast Osteoblast