Article

Journal of Cardiovascular Translational Research

, Volume 7, Issue 2, pp 192-202

First online:

Sex Differences in Translocator Protein 18 kDa (TSPO) in the Heart: Implications for Imaging Myocardial Inflammation

  • DeLisa FairweatherAffiliated withJohns Hopkins Bloomberg School of Public HealthDepartment of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health Email author 
  • , Michael J. CoronadoAffiliated withJohns Hopkins Bloomberg School of Public Health
  • , Amanda E. GartonAffiliated withJohns Hopkins Bloomberg School of Public Health
  • , Jennifer L. DziedzicAffiliated withMailman School of Public Health, Columbia University
  • , Adriana BucekAffiliated withJohns Hopkins Bloomberg School of Public Health
  • , Leslie T. CooperJr.Affiliated withMayo Clinic
  • , Jessica E. BrandtAffiliated withJohns Hopkins Bloomberg School of Public Health
  • , Fatima S. AlikhanAffiliated withMailman School of Public Health, Columbia University
  • , Haofan WangAffiliated withJohns Hopkins School of Medicine
    • , Christopher J. EndresAffiliated withJohns Hopkins School of Medicine
    • , Judy ChoiAffiliated withMailman School of Public Health, Columbia University
    • , Martin G. PomperAffiliated withJohns Hopkins School of Medicine
    • , Tomás R. GuilarteAffiliated withMailman School of Public Health, Columbia UniversityDepartment of Environmental Health Sciences, Mailman School of Public Health, Columbia University Email author 

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Abstract

Myocarditis is more severe in men than in women and difficult to diagnose due to a lack of imaging modalities that directly detect myocardial inflammation. Translocator protein 18 kDa (TSPO) is used extensively to image brain inflammation due to its presence in CD11b+ brain microglia. In this study, we examined expression of TSPO and CD11b in mice with coxsackievirus B3 (CVB3) myocarditis and biopsy sections from myocarditis patients in order to determine if it could be used to image myocarditis. We found that male mice with CVB3 myocarditis upregulated more genes associated with TSPO activation than female mice. TSPO expression was increased in the heart of male mice and men with myocarditis compared with female subjects due to testosterone, where it was expressed predominantly in CD11b+ immune cells. We show that TSPO ligands detect myocardial inflammation using microSPECT, with increased uptake of [125I]-IodoDPA-713 in male mice with CVB3 myocarditis compared with undiseased controls.

Keywords

Myocarditis Sex differences Imaging Single-photon emission computed tomography TSPO CD11b