Journal of Cardiovascular Translational Research

, Volume 6, Issue 3, pp 436–445

Beneficial Cardiac Effects of Caloric Restriction Are Lost with Age in a Murine Model of Obesity

Authors

  • Majd AlGhatrif
    • Division of Cardiology, Department of MedicineJohns Hopkins University School of Medicine
    • Division of Hospital Medicine, Bayview Department of MedicineJohns Hopkins University School of Medicine
  • Vabren L. Watts
    • Division of Cardiology, Department of MedicineJohns Hopkins University School of Medicine
  • Xiaolin Niu
    • Division of Cardiology, Department of MedicineJohns Hopkins University School of Medicine
    • Department of Cardiology, Tangdu HospitalThe Fourth Military Medical University
  • Marc Halushka
    • Department of PathologyJohns Hopkins University School of Medicine
  • Karen L. Miller
    • Division of Cardiology, Department of MedicineJohns Hopkins University School of Medicine
  • Konrad Vandegaer
    • Division of Cardiology, Department of MedicineJohns Hopkins University School of Medicine
  • Djahida Bedja
    • Department of Comparative MedicineJohns Hopkins University School of Medicine
  • Karen Fox-Talbot
    • Department of PathologyJohns Hopkins University School of Medicine
  • Alicja Bielawska
    • Department of Biochemistry and Molecular BiologyMedical University of South Carolina
  • Kathleen L. Gabrielson
    • Department of Comparative MedicineJohns Hopkins University School of Medicine
    • Division of Cardiology, Department of MedicineJohns Hopkins University School of Medicine
Article

DOI: 10.1007/s12265-013-9453-4

Cite this article as:
AlGhatrif, M., Watts, V.L., Niu, X. et al. J. of Cardiovasc. Trans. Res. (2013) 6: 436. doi:10.1007/s12265-013-9453-4

Abstract

Obesity is associated with increased diastolic stiffness and myocardial steatosis and dysfunction. The impact of aging on the protective effects of caloric restriction (CR) is not clear. We studied 2-month (younger) and 6–7-month (older)-old ob/ob mice and age-matched C57BL/6J controls (WT). Ob/ob mice were assigned to diet ad libitum or CR for 4 weeks. We performed echocardiograms, myocardial triglyceride assays, Oil Red O staining, and measured free fatty acids, superoxide, NOS activity, ceramide levels, and Western blots. In younger mice, CR restored diastolic function, reversed myocardial steatosis, and upregulated Akt phosphorylation. None of these changes was observed in the older mice; however, CR decreased oxidative stress and normalized NOS activity in these animals. Interestingly, myocardial steatosis was not associated with increased ceramide, but CR altered the composition of ceramides. In this model of obesity, aging attenuates the benefits of CR on myocardial structure and function.

Keywords

ObesityCaloric restrictionSteatosisLipotoxicityDiastolic dysfunction

Copyright information

© Springer Science+Business Media New York 2013