Genes & Nutrition

, Volume 8, Issue 1, pp 79–90

The soybean peptide lunasin promotes apoptosis of mammary epithelial cells via induction of tumor suppressor PTEN: similarities and distinct actions from soy isoflavone genistein

Authors

  • John Mark P. Pabona
    • Physiology and BiophysicsUniversity of Arkansas for Medical Sciences
    • Arkansas Children’s Nutrition Center
  • Bhuvanesh Dave
    • Arkansas Children’s Nutrition Center
    • The Methodist Hospital Research Institute
  • Ying Su
    • Arkansas Children’s Nutrition Center
    • Dana-Farber Cancer InstituteHarvard Medical School
  • Maria Theresa E. Montales
    • Physiology and BiophysicsUniversity of Arkansas for Medical Sciences
    • Arkansas Children’s Nutrition Center
  • Ben O. de Lumen
    • University of California Berkeley
  • Elvira G. de Mejia
    • University of Illinois at Urbana-Champaign
  • Omar M. Rahal
    • Arkansas Children’s Nutrition Center
    • Interdisciplinary Biomedical SciencesUniversity of Arkansas for Medical Sciences
    • Physiology and BiophysicsUniversity of Arkansas for Medical Sciences
    • Arkansas Children’s Nutrition Center
    • Interdisciplinary Biomedical SciencesUniversity of Arkansas for Medical Sciences
Research Paper

DOI: 10.1007/s12263-012-0307-5

Cite this article as:
Pabona, J.M.P., Dave, B., Su, Y. et al. Genes Nutr (2013) 8: 79. doi:10.1007/s12263-012-0307-5

Abstract

Breast cancer is the leading cause of cancer deaths in women. Diet and lifestyle are major contributing factors to increased breast cancer risk. While mechanisms underlying dietary protection of mammary tumor formation are increasingly elucidated, there remains a dearth of knowledge on the nature and precise actions of specific bioactive components present in foods with purported health effects. The 43-amino acid peptide lunasin (LUN) is found in soybeans, is bioavailable similar to the isoflavone genistein (GEN), and thus may mediate the beneficial effects of soy food consumption. Here, we evaluated whether LUN displays common and distinct actions from those of GEN in non-malignant (mouse HC11) and malignant (human MCF-7) mammary epithelial cells. In MCF-7 cells, LUN up-regulated tumor suppressor phosphatase and tensin homolog deleted in chromosome ten (PTEN) promoter activity, increased PTEN transcript and protein levels and enhanced nuclear PTEN localization, similar to that shown for GEN in mammary epithelial cells. LUN-induced cellular apoptosis, akin to GEN, was mediated by PTEN, but unlike that for GEN, was p53-independent. LUN promoted E-cadherin and β-catenin non-nuclear localization similar to GEN, but unlike GEN, did not influence the proliferative effects of oncogene Wnt1 on HC11 cells. Further, LUN did not recapitulate GEN inhibitory effects on expansion of the cancer stem-like/progenitor population in MCF-7 cells. Results suggest the concerted actions of GEN and LUN on cellular apoptosis for potential mammary tumor preventive effects and highlight whole food consumption rather than intake of specific dietary supplements with limited biological effects for greater health benefits.

Keywords

LunasinGenisteinSoyApoptosisPTENMammary epithelialBreast cancer

Copyright information

© Springer-Verlag 2012