Research Paper

Genes & Nutrition

, Volume 7, Issue 2, pp 167-178

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Differential effects of selenium and knock-down of glutathione peroxidases on TNFα and flagellin inflammatory responses in gut epithelial cells

  • G. GongAffiliated withInstitute for Cell and Molecular Biosciences, The Medical School, Newcastle University
  • , C. MéplanAffiliated withInstitute for Cell and Molecular Biosciences, The Medical School, Newcastle UniversityHuman Nutrition Research Centre, The Medical School, Newcastle University
  • , H. GautreyAffiliated withInstitute for Cell and Molecular Biosciences, The Medical School, Newcastle UniversityHuman Nutrition Research Centre, The Medical School, Newcastle University
  • , J. HallAffiliated withInstitute for Cell and Molecular Biosciences, The Medical School, Newcastle University
  • , J. E. HeskethAffiliated withInstitute for Cell and Molecular Biosciences, The Medical School, Newcastle UniversityHuman Nutrition Research Centre, The Medical School, Newcastle University Email author 

Abstract

Selenium (Se) is essential for human health. Despite evidence that Se intake affects inflammatory responses, the mechanisms by which Se and the selenoproteins modulate inflammatory signalling, especially in the gut, are not yet defined. The aim of this work was to assess effects of altered Se supply and knock-down of individual selenoproteins on NF-κB activation in gut epithelial cells. Caco-2 cells were stably transfected with gene constructs expressing luciferase linked either to three upstream NF-κB response elements and a TATA box or only a TATA box. TNFα and flagellin activated NF-κB-dependent luciferase activity and increased IL-8 expression. Se depletion decreased expression of glutathione peroxidase1 (GPX1) and selenoproteins H and W and increased TNFα-stimulated luciferase activity, endogenous IL-8 expression and reactive oxygen species (ROS) production. These effects were not mimicked by independent knock-down of either GPX1, selenoprotein H or W; indeed, GPX1 knock-down lowered TNFα-induced NF-κB activation and did not affect ROS levels. GPX4 knock-down decreased NF-κB activation by flagellin but not by TNFα. We hypothesise that Se depletion alters the pattern of expression of multiple selenoproteins that in turn increases ROS and modulates NF-κB activation in epithelial cells, but that the effect of GPX1 knock-down is ROS-independent.

Keywords

Selenoprotein Inflammatory signalling NF-κB GPx1 GPx4 Selenium