Genes & Nutrition

, 4:49

Chronic intracerebroventricular injection of TLQP-21 prevents high fat diet induced weight gain in fast weight-gaining mice

  • Alessandro Bartolomucci
  • Elena Bresciani
  • Ilaria Bulgarelli
  • Antonello E. Rigamonti
  • Tiziana Pascucci
  • Andrea Levi
  • Roberta Possenti
  • Antonio Torsello
  • Vittorio Locatelli
  • Eugenio E. Muller
  • Anna Moles
Research Paper

DOI: 10.1007/s12263-009-0110-0

Cite this article as:
Bartolomucci, A., Bresciani, E., Bulgarelli, I. et al. Genes Nutr (2009) 4: 49. doi:10.1007/s12263-009-0110-0

Abstract

The vgf gene regulates energy homeostasis and the VGF-derived peptide TLQP-21 centrally exerts catabolic effects in mice and hamsters. Here, we investigate the effect of chronic intracerebroventricular (icv) injection of TLQP-21 in mice fed high fat diet (HFD). Fast weight-gaining mice injected with the peptide or cerebrospinal fluid were selected for physiological, endocrine, and molecular analysis. TLQP-21 selectively inhibited the increase in body weight and epididymal white adipose tissue (eWAT) weight induced by HFD in control animals despite both groups having a similar degree of hyperphagia. TLQP-21 normalized the increase in leptin and decrease in ghrelin while increasing epinephrine and epinephrine/norepinephrine ratio when compared to values in controls. Finally, HFD-TLQP-21 mice showed a selective increase of eWAT β3-adrenergic receptor mRNA. Peroxisome-proliferator-activated-receptor-δ and hormone-sensing-lipase mRNA were also upregulated. In conclusion, chronic icv infusion of TLQP-21 prevented the early phase of diet-induced obesity despite overfeeding. These effects were paralleled by activation of catabolic pathways within the eWAT. Our results further support a role for TLQP-21 as a catabolic neuropeptide.

Keywords

VGF Obesity Neuropeptides White adipose tissue High fat diet 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Alessandro Bartolomucci
    • 1
    • 2
  • Elena Bresciani
    • 3
  • Ilaria Bulgarelli
    • 3
  • Antonello E. Rigamonti
    • 4
  • Tiziana Pascucci
    • 5
  • Andrea Levi
    • 6
  • Roberta Possenti
    • 6
    • 7
  • Antonio Torsello
    • 3
  • Vittorio Locatelli
    • 3
  • Eugenio E. Muller
    • 4
  • Anna Moles
    • 1
  1. 1.Institute of NeuroscienceCNRRomeItaly
  2. 2.Department of Evolutionary and Functional BiologyUniversity of ParmaParmaItaly
  3. 3.Department of Experimental and Environmental Medicine and Biotechnology, and Interdepartmental Center for Bioinformatics and ProteomicsUniversity of Milano-BicoccaMonzaItaly
  4. 4.Department of Medical Pharmacology Chemotherapy and ToxicologyUniversity of MilanoMilanItaly
  5. 5.Department of PsychologyUniversity “La Sapienza,” and I.R.C.C.S. “Fondazione Santa Lucia”RomeItaly
  6. 6.Institute of Neurobiology and Molecular NeuroscienceCNR-CERCRomeItaly
  7. 7.Department of NeuroscienceUniversity of Roma II Tor VergataRomeItaly

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