Genes & Nutrition

, Volume 2, Issue 4, pp 365–369

Effect of Cheonggukjang supplementation upon hepatic acyl-CoA synthase, carnitine palmitoyltransferase I, acyl-CoA oxidase and uncoupling protein 2 mRNA levels in C57BL/6J mice fed with high fat diet

  • Ju-Ryoun Soh
  • Dong-Hwa Shin
  • Dae Young Kwon
  • Youn-Soo Cha
Research Paper

DOI: 10.1007/s12263-007-0066-x

Cite this article as:
Soh, J., Shin, D., Kwon, D.Y. et al. Genes Nutr (2008) 2: 365. doi:10.1007/s12263-007-0066-x

Abstract

This study investigated the effect of Cheonggukjang on mRNA levels of hepatic acyl-CoA synthase (ACS), carnitine palmitoyltransferase I (CPT-I), acyl-CoA oxidase (ACO) and uncoupling protein 2 (UCP2), and on serum lipid profiles in C57BL/6J mice. Thirty male C57BL/6J mice were divided into three groups; normal diet (ND), high fat diet (HD) and high fat diet with 40% Cheonggukjang (HDC). Energy intake was significantly higher in the HDC group than in the ND and HD groups. The HDC group normalized in weight gain, epididymal and back fat (g/100 g) accumulation which are increased by high fat diet. Serum concentrations of triglyceride and total cholesterol in the HDC were significantly lower than those in the HD group. These results were confirmed by hepatic mRNA expression of enzymes and protein (ACS, CPT-1, ACO, UCP2) which is related with lipid metabolism by RT-PCR. Hepatic CPT-I, ACO and UCP2 mRNA expression was increased by Cheonggukjang supplementation. We demonstrated that Cheonggukjang supplement leads to increased mRNA expressions of enzymes and protein involved in fatty acid oxidation in liver, reduced accumulation of body fat and improvement of serum lipids in high fat diet fed mice.

Keywords

Carnitine palmitoyl transferase I (CPT-I)Fermented soybeanHigh fat dietLipid metabolismUncoupling protein 2 (UCP 2)

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Ju-Ryoun Soh
    • 1
  • Dong-Hwa Shin
    • 2
  • Dae Young Kwon
    • 3
  • Youn-Soo Cha
    • 1
  1. 1.Department of Food Science and Human Nutrition, Research Institute of Human EcologyChonbuk National UniversityJeonjuRepublic of Korea
  2. 2.Department of Food Science and TechnologyChonbuk National UniversityJeonjuRepublic of Korea
  3. 3.Division of Food Functional ResearchKorean Food Research InstitutesSongnam, Kyongki-doRepublic of Korea