Biotechnology and Bioprocess Engineering

, Volume 17, Issue 1, pp 67–75

An organic solvent-tolerant alkaline lipase from Streptomyces sp. CS268 and its application in biodiesel production

Authors

  • Poonam Mander
    • Department of Pharmacy, College of PharmacyChosun University
  • Seung Sik Cho
    • Department of Pharmacy, College of PharmacyChosun University
  • Jaya Ram Simkhada
    • Department of Pharmacy, College of PharmacyChosun University
  • Yun Hee Choi
    • Department of Pharmacy, College of PharmacyChosun University
  • Da Jeong Park
    • Department of Pharmacy, College of PharmacyChosun University
  • Jung Wan Ha
    • Department of Pharmacy, College of PharmacyChosun University
    • Department of Pharmacy, College of PharmacyChosun University
Research Paper

DOI: 10.1007/s12257-011-0347-5

Cite this article as:
Mander, P., Cho, S.S., Simkhada, J.R. et al. Biotechnol Bioproc E (2012) 17: 67. doi:10.1007/s12257-011-0347-5

Abstract

In an effort to identify a microbial lipase that can catalyze transesterification reactions used in biodiesel production, an organic solvent-tolerant lipase was purified from Streptomyces sp. CS268. The molecular weight of the purified lipase was estimated to be 37.5 kDa by SDS-PAGE. The lipase showed highest activity at a temperature of 30°C and pH 8.0 while it was stable in the pH range 4.0 ∼ 9.0 and at temperatures ≤ 50°C. It showed the highest hydrolytic activity towards medium-length acyl chain p-nitrophenyl decanoate with Km and Vmax values of 0.59 mM and 319.5 mmol/mg/min, respectively. Also, the lipase showed non-position specificity for triolein hydrolysis. The purified lipase catalyzed transesterification reaction of soybean oil with methanol, suggesting that it can be a potential enzymatic catalyst for biodiesel production.

Keywords

microbial lipaseorganic solvent-tolerantStreptomycesbiodiesel production

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2012