Biotechnology and Bioprocess Engineering

, Volume 17, Issue 1, pp 84–92

Bioconversion composition of Ssanghwa-tang fermented by Lactobacillus fermentum

Research Paper

DOI: 10.1007/s12257-011-0296-z

Cite this article as:
Yang, M.C., Kim, DS. & Ma, J.Y. Biotechnol Bioproc E (2012) 17: 84. doi:10.1007/s12257-011-0296-z


Ssanghwa-tang is a medicinal formula that is widely prescribed in Korea to decrease fatigue after an illness. Fermented herbal medicines might be made more efficacious than conventional herbal medicines by increasing the absorption and bioavailability of the active compounds. In this study, Ssanghwa-tang was fermented to produce bioconversion compositions using Lactobacillus fermentum, and six peaks were decreased, four peaks were increased and one peak newly appeared in the HPLC-DAD chromatogram. The structures of the newly-appearing compound (1) and increased (2–5) compounds were identified as follows using NMR and MS: liquiritigenin (1), nodakenetin (2), cinnamyl alcohol (3), decursinol (4), and benzoic acid (5). The decreased compounds were identified to be paeoniflorin (6), liquiritin (7), nodakenin (8), cinnamaldehyde (9), decursin (10), and decursinol angelate (11) using HPLC-DAD analysis with authentic compounds. The high performance liquid chromatography method was used to quantify the eleven constituents in Ssanghwa-tang and fermented Ssanghwa-tang. All calibration curves of the standard compounds exhibited excellent linearity with a R2 > 0.9940.


ssanghwa-tang fermentation liquiritigenin nodakenetin cinnamyl alcohol decursinol benzoic acid paeoniflorin liquiritin nodakenin cinnamaldehyde decursin decursinol angelate regression equation R2 Lactobacillus fermentum 

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Herbal Medicine Improvement Research CenterKorea Institute of Oriental MedicineDaejeonKorea

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