Biotechnology and Bioprocess Engineering

, Volume 17, Issue 1, pp 55–59

Efficient immobilization technique for enhancement of cellobiose dehydrogenase activity on silica gel

  • Eunji Kim
  • Yoon Seok Song
  • Han Suk Choi
  • Hah Young Yoo
  • Seong Woo Kang
  • Kwang Ho Song
  • Sung Ok Han
  • Seung Wook Kim
Research Paper

DOI: 10.1007/s12257-011-0085-8

Cite this article as:
Kim, E., Song, Y.S., Choi, H.S. et al. Biotechnol Bioproc E (2012) 17: 55. doi:10.1007/s12257-011-0085-8

Abstract

In this study, cellobiose dehydrogenase (CDH) of Phanerochaete chrysosporium ATCC 32629 was immobilized on silica gel for the further application of CDH in the saccharification process of biomass. To prevent the loss of enzyme activity during enzyme immobilization, the pretreatment of CDH was performed by various pretreatment materials before immobilization. When pretreated enzymes were used in immobilization, the activities of immobilized CDH were higher than non-pretreated CDH even in same amounts of immobilized protein. The specific activity of pretreated immobilized CDH with lactose was about two times higher than that of non-pretreated immobilized CDH. Moreover, the pretreated immobilized CDH showed better reusability than non-pretreated immobilized CDH, with 67.3% of its original activity being retained after 9 reuses.

Keywords

Phanerochaete chrysosporiumcellobiose dehydrogenase (CDH)immobilizationwhite-rot fungipretreatment

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Eunji Kim
    • 1
  • Yoon Seok Song
    • 1
  • Han Suk Choi
    • 1
  • Hah Young Yoo
    • 1
  • Seong Woo Kang
    • 1
  • Kwang Ho Song
    • 1
  • Sung Ok Han
    • 2
  • Seung Wook Kim
    • 1
  1. 1.Department of Chemical and Biological EngineeringKorea UniversitySeoulKorea
  2. 2.School of Life Science and BiotechnologyKorea UniversitySeoulKorea