A new microtiter plate-based screening method for microorganisms producing Alpha-amylase inhibitors
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Alpha-amylase inhibitors are widely used by the pharmaceutical and agricultural industries, such as the treatment of diabetes and obesity and insect controller. Here, we developed a colorimetric method to screen for α-amylase inhibitor producing strains or mutants with higher α-amylase inhibitor productivity. This method relies on absorbance changes at 402 nm that are due to the inhibition of α-amylase catalyzed hydrolysis of 2-Chloro-4-nitrophenyl-4-O-β-D-galactopyranosyl-maltoside by α-amylase inhibitors. The assay can be performed on a microtiter plate, making it simple and convenient. Using this method, α-amylase inhibitor producing strains and mutants with higher α-amylase inhibitor productivity can be rapidly screened. One strain, ZJB-08196, with the highest α-amylase inhibition was isolated and identified as Actinoplanes utahensis, and one mutant with higher acarbose production was obtained by screening 3,000 variants using this method.
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- A new microtiter plate-based screening method for microorganisms producing Alpha-amylase inhibitors
Biotechnology and Bioprocess Engineering
Volume 16, Issue 5 , pp 894-900
- Cover Date
- Print ISSN
- Online ISSN
- The Korean Society for Biotechnology and Bioengineering
- Additional Links
- α-amylase inhibitors
- human salivary amylase
- diabetes mellitus
- Industry Sectors