Biotechnology and Bioprocess Engineering

, Volume 16, Issue 3, pp 482–487

Exploring low-cost carbon sources for microbial lipids production by fed-batch cultivation of Cryptococcus albidus

Authors

  • Qiang Fei
    • Biochemical Engineering Lab, Department of Chemical and Biomolecule EngineeringKAIST
    • Biochemical Engineering Lab, Department of Chemical and Biomolecule EngineeringKAIST
    • Eews BiotechKAIST
  • Longan Shang
    • College of Biological and Chemical Engineering, Ningbo Institute of TechnologyZhejiang University
  • Jin-dal-rae Choi
    • Biochemical Engineering Lab, Department of Chemical and Biomolecule EngineeringKAIST
Research Paper

DOI: 10.1007/s12257-010-0370-y

Cite this article as:
Fei, Q., Chang, H.N., Shang, L. et al. Biotechnol Bioproc E (2011) 16: 482. doi:10.1007/s12257-010-0370-y

Abstract

Volatile fatty acids (VFAs), acetic acid, acetates, and ethanol were used as carbon sources for the production of microbial lipids using Cryptococcus albidus in batch cultures. C. albidus utilized organic acids less than glucose in the production of lipids, resulting in a lipid yield coefficient on VFAs of 0.125 g/g. In a two-stage batch culture, the lipid content increased to 43.8% (w/w) when VFAs were used as the sole carbon source in the second stage, which was two times higher than that of the batch culture. Furthermore, a 192 h, two-stage fed-batch cultivation of C. albidus produced a dry cell weight, lipid concentration, and lipid content of 26.4 g/L, 14.5 g/L, and 55.1% (w/w), respectively. The fed-batch culture model used in this study featured pure VFA solutions, with intermittent feeding, under oxygen-enriched air supply conditions. This study investigated several alternative carbon sources to reduce the cost of microbial lipids production and proved the feasibility of using VFAs as the carbon source for the provision of a high lipid content and productivity.

Keywords

microbial lipids productionCryptococcus albiduslow-cost carbon sourcetwo-stage cultivationoxygen-enriched air supplybiodiesel

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2011