Biotechnology and Bioprocess Engineering

, Volume 14, Issue 6, pp 788-794

First online:

Production of milk clotting protease by a local isolate of Mucor circinelloides under SSF using agro-industrial wastes

  • R. SathyaAffiliated withDepartment of Microbiology, School of Life Sciences, Karpagam University
  • , B. V. PradeepAffiliated withDepartment of Microbiology, School of Life Sciences, Karpagam University
  • , J. AngayarkanniAffiliated withDepartment of Microbial Biotechnology, Bharathiar University
  • , M. PalaniswamyAffiliated withDepartment of Microbiology, School of Life Sciences, Karpagam University Email author 

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Agro-industrial residues, a cheap source of energy have high potential in the area of fermentation for the production of enzymes. Twenty agro-industrial residues were evaluated to check the possibility of potential utilization of substrates in SSF for milk clotting enzyme protease production by Mucor circinelloides. In this study, dhal husk holds the greatest promise for cost effective production of the milk clotting enzyme. The dhal husk supported maximum milk clotting protease production, and yield was improved with the supplementation of sucrose and yeast extract as carbon and nitrogen source, respectively. Among all the physico-chemical parameters tested, the best results were obtained in a medium having moisture content of 20% at pH 7.0, when inoculated with 30% of spore suspension and incubated at 30°C for 5 days. The activity was increased further on addition of Ca2+, Cu2+, and Mg2+ ions. The purified milk-clotting protease obtained from M. circinelloides was successfully applied and compared with commercial rennet in the manufacture of a cheddar cheese.


dhal husk agro-industrial residues milk-clotting protease solid-state fermentation cheese production