Biotechnology and Bioprocess Engineering

, Volume 13, Issue 4, pp 401–409

Long-term operation of depth filter perfusion systems (DFPS) for monoclonal antibody production using recombinant CHO cells: Effect of temperature, pH, and dissolved oxygen

Article

DOI: 10.1007/s12257-008-0155-8

Cite this article as:
Lee, J.C., Kim, D.Y., Oh, D.J. et al. Biotechnol Bioproc E (2008) 13: 401. doi:10.1007/s12257-008-0155-8

Abstract

Recombinant CHO cells of DG44 origin (CS*13-1.00), expressing a chimeric antibody against the S surface antigen of the Hepatitis B virus, were cultivated in single-stage and two-stage depth filter perfusion systems (DFPS) under varying temperature, pH, and oxygen tension conditions to determine their effects on recombinant antibody production. A long-term culture was carried out in a single-stage depth filter for 81 days, during which an occasional clog interrupted the experiment. However, this problem was solved via trypsin injection. The DFPS showed a steady production of monoclonal antibody at a concentration of 100∼150 mg/L. As the cultivation temperature was increased from 33 to 37°C, the monoclonal antibody (Mab) concentration increased from 80.33 to 133.47 mg/L. Likewise, the glucose uptake rate (GUR) and lactate production rate (LPR) also increased. With an increase in pH from 6.95 to 7.61, the Mab concentration increased from 61.64 to 94.31 mg/L. When the oxygen tension was increased from 60 to 80%, the Mab concentration increased from 93.78 to 128.30 mg/L.

Keywords

Chinese hamster ovary cellsperfusion culturerecombinant antibodytwo-stage depth filter perfusion system

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg GmbH 2008

Authors and Affiliations

  1. 1.Department of Chemical and Biomolecular EngineeringKAISTDaejeonKorea
  2. 2.Department of Bioscience and BiotechnologySejong UniversitySeoulKorea
  3. 3.Center for Advanced Bioseparation TechnologyInha universityIncheonKorea
  4. 4.Korea Biotechnology Commercialization CenterKorea Institute of Industrial TechnologyIncheonKorea