Biotechnology and Bioprocess Engineering

, Volume 13, Issue 4, pp 401–409

Long-term operation of depth filter perfusion systems (DFPS) for monoclonal antibody production using recombinant CHO cells: Effect of temperature, pH, and dissolved oxygen

Authors

  • Joon Chul Lee
    • Department of Chemical and Biomolecular EngineeringKAIST
    • Department of Bioscience and BiotechnologySejong University
  • Do Yun Kim
    • Department of Chemical and Biomolecular EngineeringKAIST
    • Department of Bioscience and BiotechnologySejong University
    • Department of Bioscience and BiotechnologySejong University
    • Center for Advanced Bioseparation TechnologyInha university
    • Department of Chemical and Biomolecular EngineeringKAIST
Article

DOI: 10.1007/s12257-008-0155-8

Cite this article as:
Lee, J.C., Kim, D.Y., Oh, D.J. et al. Biotechnol Bioproc E (2008) 13: 401. doi:10.1007/s12257-008-0155-8
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Abstract

Recombinant CHO cells of DG44 origin (CS*13-1.00), expressing a chimeric antibody against the S surface antigen of the Hepatitis B virus, were cultivated in single-stage and two-stage depth filter perfusion systems (DFPS) under varying temperature, pH, and oxygen tension conditions to determine their effects on recombinant antibody production. A long-term culture was carried out in a single-stage depth filter for 81 days, during which an occasional clog interrupted the experiment. However, this problem was solved via trypsin injection. The DFPS showed a steady production of monoclonal antibody at a concentration of 100∼150 mg/L. As the cultivation temperature was increased from 33 to 37°C, the monoclonal antibody (Mab) concentration increased from 80.33 to 133.47 mg/L. Likewise, the glucose uptake rate (GUR) and lactate production rate (LPR) also increased. With an increase in pH from 6.95 to 7.61, the Mab concentration increased from 61.64 to 94.31 mg/L. When the oxygen tension was increased from 60 to 80%, the Mab concentration increased from 93.78 to 128.30 mg/L.

Keywords

Chinese hamster ovary cellsperfusion culturerecombinant antibodytwo-stage depth filter perfusion system
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© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg GmbH 2008