Biotechnology and Bioprocess Engineering

, Volume 14, Issue 2, pp 220–224

Optimization of process variables in castor oil hydrolysis by Candida rugosa lipase with buffer as dispersion medium

  • Debajyoti Goswami
  • Jayanta Kumar Basu
  • Sirshendu De
Articles

DOI: 10.1007/s12257-008-0123-3

Cite this article as:
Goswami, D., Basu, J.K. & De, S. Biotechnol Bioproc E (2009) 14: 220. doi:10.1007/s12257-008-0123-3

Abstract

In this study, castor oil is hydrolyzed in presence of Candida rugosa lipase, while in the buffer (aqueous) phase as a dispersion medium. The following conditions were used to optimize the process: speed of agitation, initial pH of buffer phase, temperature, and ratio of buffer phase volume to oil weight. The optimal conditions are 1,100 rpm, pH 6.5, temperature 35°C, and 3:1 buffer phase volume to oil weight ratio. Under these described conditions, the reusability of lipase was tested and it was found that nearly 80% of original hydrolysis percentage was achieved after the first recycle.

Keywords

castor oilcandida rugosa lipasehydrolysisricinoleic acid

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg GmbH 2009

Authors and Affiliations

  • Debajyoti Goswami
    • 1
  • Jayanta Kumar Basu
    • 1
  • Sirshendu De
    • 1
  1. 1.Department of Chemical EngineeringIndian Institute of Technology KharagpurKharagpurIndia