Articles

Biotechnology and Bioprocess Engineering

, Volume 14, Issue 2, pp 220-224

Optimization of process variables in castor oil hydrolysis by Candida rugosa lipase with buffer as dispersion medium

  • Debajyoti GoswamiAffiliated withDepartment of Chemical Engineering, Indian Institute of Technology Kharagpur
  • , Jayanta Kumar BasuAffiliated withDepartment of Chemical Engineering, Indian Institute of Technology Kharagpur
  • , Sirshendu DeAffiliated withDepartment of Chemical Engineering, Indian Institute of Technology Kharagpur Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

In this study, castor oil is hydrolyzed in presence of Candida rugosa lipase, while in the buffer (aqueous) phase as a dispersion medium. The following conditions were used to optimize the process: speed of agitation, initial pH of buffer phase, temperature, and ratio of buffer phase volume to oil weight. The optimal conditions are 1,100 rpm, pH 6.5, temperature 35°C, and 3:1 buffer phase volume to oil weight ratio. Under these described conditions, the reusability of lipase was tested and it was found that nearly 80% of original hydrolysis percentage was achieved after the first recycle.

Keywords

castor oil candida rugosa lipase hydrolysis ricinoleic acid