Biotechnology and Bioprocess Engineering

, Volume 13, Issue 5, pp 560–565

Two-stage depth filter perfusion culture for recombinant antibody production by recombinant Chinese hamster ovary cell

Article

DOI: 10.1007/s12257-008-0003-x

Cite this article as:
Lee, J.C., Kim, D.Y., Oh, D.J. et al. Biotechnol Bioproc E (2008) 13: 560. doi:10.1007/s12257-008-0003-x

Abstract

A rCHO cell line of DUKX origin 26*-320, producing recombinant antibody against the human platelet, was cultivated in a two-stage depth filter perfusion system (DFPS) for 20 days in order to attain high recombinant antibody concentration. The productivity of the first stage DFPS bioreactor reached 53 times that of the batch culture in a controlled stirred tank reactor and was showed 12.1 mg/L antibody concentration at a perfusion rate of 6.0 d−1. Glucose concentration in the first DFPS was maintained at 1.5 g/L to avoid cell damage in the perfusion culture. A second stage DFPS system was attached to the first DFPS, which resulted in a low glucose concentration of 0.02 g/L and a high antibody concentration of 23.9 mg/L. The two-stage depth filter perfusion culture yielded 60% higher product concentration than the batch and 49-fold higher productivity of 69.3 mg/L/d in comparison with that (1.4 mg/L/d) in a batch system. Furthermore, antibody concentration of the second stage was 97% higher than that of the first stage, and the antibody productivities were comparable to that of the first stage. This two-stage DFPS system also showed potential for higher titer production of recombinant antibody and high volumetric productivity for long-term culture of bio-pharmaceutical substances.

Keywords

CHO cellsperfusion culturerecombinant antibodytwo-stage depth filter perfusion system

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg GmbH 2008

Authors and Affiliations

  1. 1.Department of Chemical and Biomolecular EngineeringKAISTDaejeonKorea
  2. 2.Department of Bioscience and BiotechnologySejong UniversitySeoulKorea
  3. 3.Center for Advanced Bioseparation TechnologyInha UniversityIncheonKorea
  4. 4.Korea Institute of Industrial TechnologyIncheonKorea