Pathology & Oncology Research

, Volume 20, Issue 3, pp 747–754

Characterization of the Attenuation of Breast Cancer Bone Metastasis in Mice by Zoledronic Acid Using 99mTc bone Scintigraphy

Authors

  • Chunyu Wu
    • Department of Breast Surgery, Longhua HospitalShanghai University of Traditional Chinese Medicine
  • Shunfang Yang
    • Department of Nuclear Medicine, Shanghai Chest HospitalShanghai JiaoTong University
  • Zhenping Sun
    • Department of Breast Surgery, Longhua HospitalShanghai University of Traditional Chinese Medicine
  • Xianghui Han
    • Pharmacology Laboratory of Traditional Chinese Medicine, Longhua HospitalShanghai University of Traditional Chinese Medicine
  • Yiyi Ye
    • Pharmacology Laboratory of Traditional Chinese Medicine, Longhua HospitalShanghai University of Traditional Chinese Medicine
    • Department of Breast Surgery, Longhua HospitalShanghai University of Traditional Chinese Medicine
    • Pharmacology Laboratory of Traditional Chinese Medicine, Longhua HospitalShanghai University of Traditional Chinese Medicine
    • Department of Breast Surgery and Pharmacology Laboratory of Traditional Chinese Medicine, Long Hua HospitalShanghai University of Traditional Chinese Medicine
Research

DOI: 10.1007/s12253-014-9756-z

Cite this article as:
Wu, C., Yang, S., Sun, Z. et al. Pathol. Oncol. Res. (2014) 20: 747. doi:10.1007/s12253-014-9756-z
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Abstract

Metastatic breast cancer often metastasizes to bone. The purposes of the study were (1) to evaluate the use of 99mTc-MDP bone scintigraphy for detection of metastatic bone lesions, and (2) to determine the efficacy of zoledronic acid in mice with breast cancer bone metastasis. All tumor-bearing mice were analyzed with radionuclide bone scintigraphy, X-ray, and histological analysis. The metastatic bone tissue was also harvested and analyzed by western blotting and real-time qPCR. Interestingly, zoledronic acid significantly decreased both the tumor burden and the incidence of bone metastasis in mice. In addition, histomorphometric, stereological, and molecular biology analyses demonstrated that zoledronic acid may function to inhibit breast cancer cell growth in the bone microenvironment and regulate the function of osteoblasts and osteoclasts in tumor-bearing mice. Finally, the attenuation of breast cancer bone metastasis using zoledronic acid can be accurately characterized by 99mTc bone scintigraphy in mice.

Keywords

Bone metastasisBreast cancer99mTc-MDP bone scintigraphyZoleronic acid

Copyright information

© Arányi Lajos Foundation 2014