Pathology & Oncology Research

, Volume 19, Issue 3, pp 521–527

A-Kinase Anchoring Proteins 10 Expression in Relation to 2073A/G Polymorphism and Tumor Progression in Patients with Colorectal Cancer

Authors

  • Mojin Wang
    • Department of Gastrointestinal SurgeryInstitute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan University
  • Dan Zhang
    • Department of Gastrointestinal SurgeryInstitute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan University
  • Rui Wang
    • Department of GastroenterologyWest China Hospital, Sichuan University
  • Yuanyi Rui
    • Department of Gastrointestinal SurgeryInstitute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan University
  • Jin Zhou
    • Department of Gastrointestinal SurgeryInstitute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan University
  • Rong Wang
    • Department of Gastrointestinal SurgeryInstitute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan University
  • Bin Zhou
    • Department of Gastrointestinal SurgeryInstitute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan University
  • Xiaoran Huang
    • Department of PathologyWest China Hospital, Sichuan University
  • Lie Yang
    • Department of Gastrointestinal SurgeryInstitute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan University
  • Yuan Li
    • Department of Pediatric SurgeryInstitute of Digestive Surgery and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University
  • Jiankun Hu
    • Department of Gastrointestinal SurgeryInstitute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan University
    • Department of Gastrointestinal SurgeryInstitute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan University
  • Xiaofeng Sun
    • Division of Oncology, Department of Clinical and Experimental MedicineFaculty of Health Sciences, University of Linköping
Research

DOI: 10.1007/s12253-013-9612-6

Cite this article as:
Wang, M., Zhang, D., Wang, R. et al. Pathol. Oncol. Res. (2013) 19: 521. doi:10.1007/s12253-013-9612-6

Abstract

The cAMP/PKA signalling events regulated by A-kinase anchoring proteins 10 (AKAP10) is involved in tumorigenesis. Previous study showed that AKAP10 polymorphism (2073 A/G, I646V) was associated with colorectal cancer risk. However, there was no literature reporting the role of AKAP10 in the pathogenesis of colorectal cancer. The aim of the study was to investigate the clinicopathologic significance of A-kinase anchoring proteins 10 (AKAP 10) expression and the relationship with its polymorphism in colorectal cancer. The expression of AKAP10 was determined by immunohistochemical staining (IHC) and western blot assay on colorectal cancer (n = 176), adenoma (n = 87) and distant normal mucosa (n = 72). 176 patients with colorectal cancer were genotyped for AKAP10 2073A/G polymorphism by TaqMan RT-PCR. We found that the positive expression rate of AKAP10 in colorectal cancer (59 %) was significantly higher than those in adenoma (39 %) and distant normal mucosa (42 %) (P = 0.004). There was no significant difference between adenoma and distant normal mucosa (P = 0.741). Positive AKAP10 staining was correlated with deeper tumor invasion (P < 0.001), lymph nodes metastasis (P = 0.022), advanced tumor stage (P < 0.001) and poorly differentiated degree (P = 0.003). Compared with AA genotype (52 %), positive expression of AKAP10 was significantly increased in colorectal cancer patients with the variant (AG+GG) genotypes (68 %, P = 0.033). It was concluded that AKAP10 may play an important role in the development and progression of colorectal cancer.

Keywords

A-kinase anchoring proteins 10Immunohistochemical stainingWestern blotPolymorphismColorectal cancer

Copyright information

© Arányi Lajos Foundation 2013