Pathology & Oncology Research

, Volume 18, Issue 4, pp 895–902

The Plasmodium Circumsporozoite Protein, a Novel NF-κB Inhibitor, Suppresses the Growth of SW480

  • Yan Ding
  • Xiaobing Huang
  • Taiping Liu
  • Yong Fu
  • Zhangping Tan
  • Hong Zheng
  • Taoli Zhou
  • Jigang Dai
  • Wenyue Xu
Research

DOI: 10.1007/s12253-012-9519-7

Cite this article as:
Ding, Y., Huang, X., Liu, T. et al. Pathol. Oncol. Res. (2012) 18: 895. doi:10.1007/s12253-012-9519-7

Abstract

The blocking of NF-κB activation is a promising strategy for the treatment of colorectal cancer. The circumsporozoite protein (CSP), a key component of the sporozoite stage of the malaria parasite, was recently reported to block NF-κB activation in hepatocytes. Thus, we investigated the effect of the CSP on the growth of the human colon cancer cell line, SW480. We demonstrated that transfection with a recombinant plasmid expressing CSP inhibited the proliferation of SW480 in a dose-dependent manner and induced the apoptosis of SW480. A NF-κB gene reporter assay showed that both the CSP and its nuclear localization signal (NLS) motif could equally suppress the activation of NF-κB following the stimulation with human recombinant TNF-α in the SW480. Furthermore, western blot analysis indicated that NLS did not affect the phosphorylation and degradation of IκB, but could sharply inhibit the nuclear translocation of NF-κB in TNF-α stimulated SW480. Hence, our data suggest that the CSP might be explored as a new NF-κB inhibitor for the treatment of colorectal cancer.

Keyword

Circumsporozoite proteinNuclear transcription factor κBHuman colon cancer cell lineProliferationApoptosis

Copyright information

© Arányi Lajos Foundation 2012

Authors and Affiliations

  • Yan Ding
    • 1
  • Xiaobing Huang
    • 2
  • Taiping Liu
    • 1
  • Yong Fu
    • 1
  • Zhangping Tan
    • 1
  • Hong Zheng
    • 1
  • Taoli Zhou
    • 1
  • Jigang Dai
    • 3
  • Wenyue Xu
    • 1
  1. 1.Department of Pathogenic BiologyThird Military Medical UniversityChongqingPeople’s Republic of China
  2. 2.Department of Hepatobiliary Surgery, Xinqiao HospitalThird Military Medical UniversityChongqingPeople’s Republic of China
  3. 3.Department of Thoracic Surgery of Xinqiao HospitalThe Third Military Medical UniversityChongqingPeople’s Republic of China