Pathology & Oncology Research

, Volume 18, Issue 4, pp 895–902

The Plasmodium Circumsporozoite Protein, a Novel NF-κB Inhibitor, Suppresses the Growth of SW480

Authors

  • Yan Ding
    • Department of Pathogenic BiologyThird Military Medical University
  • Xiaobing Huang
    • Department of Hepatobiliary Surgery, Xinqiao HospitalThird Military Medical University
  • Taiping Liu
    • Department of Pathogenic BiologyThird Military Medical University
  • Yong Fu
    • Department of Pathogenic BiologyThird Military Medical University
  • Zhangping Tan
    • Department of Pathogenic BiologyThird Military Medical University
  • Hong Zheng
    • Department of Pathogenic BiologyThird Military Medical University
  • Taoli Zhou
    • Department of Pathogenic BiologyThird Military Medical University
    • Department of Thoracic Surgery of Xinqiao HospitalThe Third Military Medical University
    • Department of Pathogenic BiologyThird Military Medical University
Research

DOI: 10.1007/s12253-012-9519-7

Cite this article as:
Ding, Y., Huang, X., Liu, T. et al. Pathol. Oncol. Res. (2012) 18: 895. doi:10.1007/s12253-012-9519-7

Abstract

The blocking of NF-κB activation is a promising strategy for the treatment of colorectal cancer. The circumsporozoite protein (CSP), a key component of the sporozoite stage of the malaria parasite, was recently reported to block NF-κB activation in hepatocytes. Thus, we investigated the effect of the CSP on the growth of the human colon cancer cell line, SW480. We demonstrated that transfection with a recombinant plasmid expressing CSP inhibited the proliferation of SW480 in a dose-dependent manner and induced the apoptosis of SW480. A NF-κB gene reporter assay showed that both the CSP and its nuclear localization signal (NLS) motif could equally suppress the activation of NF-κB following the stimulation with human recombinant TNF-α in the SW480. Furthermore, western blot analysis indicated that NLS did not affect the phosphorylation and degradation of IκB, but could sharply inhibit the nuclear translocation of NF-κB in TNF-α stimulated SW480. Hence, our data suggest that the CSP might be explored as a new NF-κB inhibitor for the treatment of colorectal cancer.

Keyword

Circumsporozoite proteinNuclear transcription factor κBHuman colon cancer cell lineProliferationApoptosis

Copyright information

© Arányi Lajos Foundation 2012