Virologica Sinica

, Volume 27, Issue 6, pp 344–351

Immunogenic and protective properties of the first kazakhstan vaccine against pandemic influenza A (H1N1) pdm09 in Ferrets

Authors

    • Research Institute for Biological Safety Problems (RIBSP)
  • Zhailaubai Kydyrbayev
    • Research Institute for Biological Safety Problems (RIBSP)
  • Abylai Sansyzbay
    • Research Institute for Biological Safety Problems (RIBSP)
  • Berik Khairullin
    • Research Institute for Biological Safety Problems (RIBSP)
  • Sholpan Ryskeldinova
    • Research Institute for Biological Safety Problems (RIBSP)
  • Nurika Assanzhanova
    • Research Institute for Biological Safety Problems (RIBSP)
  • Yerken Kozhamkulov
    • Research Institute for Biological Safety Problems (RIBSP)
  • Dulat Inkarbekov
    • Research Institute for Biological Safety Problems (RIBSP)
Research Article

DOI: 10.1007/s12250-012-3272-7

Cite this article as:
Tabynov, K., Kydyrbayev, Z., Sansyzbay, A. et al. Virol. Sin. (2012) 27: 344. doi:10.1007/s12250-012-3272-7

Abstract

This paper presents the results of a pre-clinical study of the immunogenicity and efficacy of an egg-derived, inactivated, whole-virion adjuvanted vaccine (Refluvac®) on ferret models. For this purpose, groups of eight ferrets (6 to 7 months old) were injected with 0.5 mL of vaccine specimens containing 3.75, 7.5 or 15.0 μg of virus hemagglutinin. Administration was intramuscular and given either as a single dose or as two doses 14 days apart. All vaccine specimens manifested immunogenicity in ferrets for single (HI titer, from 51 ± 7 to 160 ± 23) and double (HI titer, from 697 ± 120 to 829 ± 117) administrations. To assess the protective effects of the vaccine, ferrets from the vaccinated and control groups were infected intranasally with pandemic virus A/California/7/09 (H1N1) pdm09 at a dose of 106 EID50/0.5 mL. Fourteen days post-infection, the ferrets inoculated with single or double vaccines containing 3.75, 7.5 or 15.0 μg of hemagglutinin per dose showed no signs of influenza infection, weight loss, or body temperature rise, and no premature deaths occurred. The number of vaccinated ferrets shedding the virus via the upper airway, as well as the amount of virus shed after infection, was significantly reduced in comparison with animals from the control group. Based on our results, we suggest that a single vaccination at a dose of 3.75 or 7.5 μg hemagglutinin be used for Phase I clinical trials.

Keywords

VaccinePandemic Influenza A (H1N1) pdm09Immunogenic and Protective PropertiesFerrets

Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2012