Virologica Sinica

, 26:156

Characterization of the receptor-binding domain of Ebola glycoprotein in viral entry

Authors

  • Jizhen Wang
    • Department of Microbiology and Immunology, College of MedicineUniversity of Illinois at Chicago
  • Balaji Manicassamy
    • Department of Microbiology and Immunology, College of MedicineUniversity of Illinois at Chicago
  • Michael Caffrey
    • Department of Biochemistry and Molecular Genetics, College of MedicineUniversity of Illinois at Chicago
    • Department of Microbiology and Immunology, College of MedicineUniversity of Illinois at Chicago
Article

DOI: 10.1007/s12250-011-3194-9

Cite this article as:
Wang, J., Manicassamy, B., Caffrey, M. et al. Virol. Sin. (2011) 26: 156. doi:10.1007/s12250-011-3194-9

Abstract

Ebola virus infection causes severe hemorrhagic fever in human and non-human primates with high mortality. Viral entry/infection is initiated by binding of glycoprotein GP protein on Ebola virion to host cells, followed by fusion of virus-cell membrane also mediated by GP. Using an human immunodeficiency virus (HIV)-based pseudotyping system, the roles of 41 Ebola GP1 residues in the receptor-binding domain in viral entry were studied by alanine scanning substitutions. We identified that four residues appear to be involved in protein folding/structure and four residues are important for viral entry. An improved entry interference assay was developed and used to study the role of these residues that are important for viral entry. It was found that R64 and K95 are involved in receptor binding. In contrast, some residues such as I170 are important for viral entry, but do not play a major role in receptor binding as indicated by entry interference assay and/or protein binding data, suggesting that these residues are involved in post-binding steps of viral entry. Furthermore, our results also suggested that Ebola and Marburg viruses share a common cellular molecule for entry.

Key words

Receptor-binding domainEbola virusGlycoproteinViral Entry
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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2011