Article

Virologica Sinica

, Volume 25, Issue 6, pp 440-444

First online:

PrP 106-126 Altered PrP mRNA gene expression in mouse microglia BV-2 Cells

  • Yu BaiAffiliated withThe state key Lab of Agrobiotechnology National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University
  • , Yu-rong LiAffiliated withDepartment of Animal Science and Technology, Agricultural University of Hebei
  • , Gui-hua WangAffiliated withDepartment of Animal Science and Technology, Agricultural University of Shandong
  • , Xiang-mei ZhouAffiliated withThe state key Lab of Agrobiotechnology National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University
  • , De-ming ZhaoAffiliated withThe state key Lab of Agrobiotechnology National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University Email author 

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Abstract

Prion diseases are infectious and fatal neurodegenerative diseases. The pathogenic agent is an abnormal prion protein aggregate. Microglial activation in the centre nervous system is a characteristic feature of prion disease. In this study, we examined the effect of PrP 106–126 on PrP mRNA gene expression in Mouse microglia cells BV-2 by real-time quantitative PCR. PrP mRNA expression level was found to be significantly increased after 18 h exposure of BV-2 cells to PrP 106–126, with 3-fold increase after 18 h and 4.5-fold increase after 24 h and BV-2 cells proliferating occurred correspondingly. Our results provide the first in vitro evidence of the increase of PrP mRNA levels in microglial cells exposed to PrP 106–126, and indicate that microglial cells might play a critical role in prion pathogenesis.

Key words

Prion PrP106-126 PrP mRNA Mouse microglia BV-2 Cells