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R2-like Gene Contributes to Resistance to Phytophthora infestans in Polish Potato Cultivar Bzura

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Abstract

Potato cultivar Bzura bred in 1986 is one of the few European cultivars outstanding in high level of durable resistance to late blight, but its resistance has not been characterized. The presented study was aimed at clarifying bases of durable resistance in Bzura by testing its resistance and resistance in progeny individuals. In ten-year field experiment Bzura was significantly more resistant than mid-resistant standards Escort and Robijn, and only slightly less resistant than highly resistant Sárpo Mira. Bzura expressed also high resistance in detached leaflet tests when inoculated with specific isolates of Phytophthora infestans. It was found in Bzura progeny that this race-specific resistance segregated in 1:1 ratio and is governed by a major resistance gene R2-like. However, the long-lasting field resistance observed in Bzura could be explained by combination of R2-like gene and specific genetic background of this cultivar rather than by this gene exclusively.

Resumen

La variedad de papa Bzura, de 1986, es una de las pocas variedades europeas sobresalientes en alto nivel de resistencia durable al tizón tardío, pero no se ha caracterizado su resistencia. El presente estudio tuvo como propósito la clarificación de las bases de la resistencia durable en Bzura mediante la prueba de su resistencia y de la de individuos de su progenie. En experimentos de campo de diez años Bzura fue significativamente más resistente que las de resistencia media estándar Escort y Robijn, y solo ligeramente menos resistente que la altamente resistente Sárpo Mira. Bzura expresó también alta resistencia en pruebas de hoja separada cuando se le inoculó con aislamientos específicos de Phytophthora infestans. Se encontró en la progenie de Bzura que esta resistencia específica a razas segregó en una proporción de 1:1 y está gobernada por un gene mayor de resistencia tipo R2. No obstante, la resistencia durable de campo observada en Bzura pudiera explicarse por la combinación de un gene tipo R2 y un respaldo genético específico de esta variedad, en vez de este gene exclusivamente.

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Acknowledgments

The authors wish to thank Sylwester Sobkowiak for preparing P. infestans cultures and suspensions for detached leaflet tests and Emil Stefańczyk for help in preparing samples for sequencing and sequence data analyses. This study was partly financed be Polish Ministry of Science and Higher Education / National Science Center, grant N N310 162738. Sequencing of the R2-like gene homolog from potato cv. Bzura was funded from Norway Grants in the Polish-Norwegian Research Programme operated by the National Centre for Research and Development, grant POTPAT, number: Pol-Nor/202448/28/2013.

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  1. Plant Breeding and Acclimatization Institute – National Research Institute, Młochów Research Center, Platanowa Str. 19, Młochów, 05-831, Poland

    Jarosław Plich, Beata Tatarowska, Renata Lebecka, Jadwiga Śliwka, Ewa Zimnoch-Guzowska & Bogdan Flis

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  1. Jarosław Plich
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Plich, J., Tatarowska, B., Lebecka, R. et al. R2-like Gene Contributes to Resistance to Phytophthora infestans in Polish Potato Cultivar Bzura. Am. J. Potato Res. 92, 350–358 (2015). https://doi.org/10.1007/s12230-015-9437-9

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