Folia Microbiologica

, Volume 59, Issue 3, pp 217–222

Oxidative stress in cystic fibrosis patients with Burkholderia cenocepacia airway colonization: relation of 8-isoprostane concentration in exhaled breath condensate to lung function decline

  • Libor Fila
  • Alžběta Grandcourtová
  • Jaroslav Chládek
  • Jaromír Musil
Article

DOI: 10.1007/s12223-013-0285-z

Cite this article as:
Fila, L., Grandcourtová, A., Chládek, J. et al. Folia Microbiol (2014) 59: 217. doi:10.1007/s12223-013-0285-z

Abstract

The association between oxidative stress and neutrophilic inflammation in cystic fibrosis (CF) lung disease is well recognized. 8-Isoprostane is a product of non-enzymatic oxidation of arachidonic acid. The aim of the present study was to examine the relationship between lung function decline and 8-isoprostane concentrations in exhaled breath condensate (EBC) in CF patients with Burkholderia cenocepacia airway colonization. Concentrations of 8-isoprostane in EBC were measured in 24 stable CF patients with B. cenocepacia airway colonization. The median (interquartile range) age of the cohort was 23.9 (22.0; 26.6) years. All patients underwent clinical examinations and pulmonary function tests at the time of EBC collection and in 1-, 3-, and 5-year intervals. 8-Isoprostane concentrations in EBC correlated to 1- and 3-year declines of forced expiratory volume in 1 s (FEV1) with rS values of −0.511 (p = 0.0011) and −0.495 (p = 0.016), respectively. In multiple regression analysis, 8-isoprostane concentrations in EBC were the only independent predictor for 1-year FEV1 decline (p = 0.01). When the median value of 8-isoprostane concentration in EBC (10.0 pg/mL) was used as a cutoff, subgroups of patients with lower and higher level of oxidative stress had significantly different median (interquartile range) FEV1 declines in 1-year interval, −2.4 % (−5.3; 0.8) and −7.3 % (−10.3; −5.8) predicted (p = 0.009). In conclusion, 8-isoprostane concentrations in EBC correlated to short-term lung function decline in CF patients with B. cenocepacia airway colonization. This correlation reflects the role of oxidative stress in CF lung pathogenesis and contributes to prediction of prognosis in these patients.

Abbreviations

ATS

American Thoracic Society

CF

Cystic fibrosis

EBC

Exhaled breath condensate

ERS

European Respiratory Society

FEV1

Forced expiratory volume in 1 s

IQR

Interquartile range

ROS

Reactive oxygen species

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2013

Authors and Affiliations

  • Libor Fila
    • 1
  • Alžběta Grandcourtová
    • 1
  • Jaroslav Chládek
    • 2
  • Jaromír Musil
    • 3
  1. 1.Department of Pneumology, Charles University, 2nd Faculty of MedicineUniversity Hospital MotolPragueCzech Republic
  2. 2.Department of PharmacologyCharles University, Faculty of Medicine in Hradec KrálovéHradec KralovéCzech Republic
  3. 3.Pulmonary DepartmentHospital MělníkMělníkCzech Republic