Folia Microbiologica

, Volume 58, Issue 2, pp 111–121

Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus

Authors

  • Dana Cechova
    • First Faculty of Medicine, Institute of Immunology and MicrobiologyCharles University in Prague
  • Michaela Novakova
    • First Faculty of Medicine, Institute of Immunology and MicrobiologyCharles University in Prague
  • Karel Mikulik
    • Institute of MicrobiologyAcademy of Sciences of the Czech Republic
  • Olga Novotna
    • First Faculty of Medicine, Institute of Immunology and MicrobiologyCharles University in Prague
  • Jaroslav Julak
    • First Faculty of Medicine, Institute of Immunology and MicrobiologyCharles University in Prague
  • Peter Zanvit
    • First Faculty of Medicine, Institute of Immunology and MicrobiologyCharles University in Prague
    • First Faculty of Medicine, Institute of Immunology and MicrobiologyCharles University in Prague
Article

DOI: 10.1007/s12223-012-0181-y

Cite this article as:
Cechova, D., Novakova, M., Mikulik, K. et al. Folia Microbiol (2013) 58: 111. doi:10.1007/s12223-012-0181-y
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Abstract

Mucosal immunization with non-living antigens usually requires the use of an adjuvant. The adjuvant activity of Bacillus firmus in the mucosal immunization of mice was described by our laboratory previously. In the present study, subcellular localization of B. firmus activities was followed. After mechanical disintegration, subcellular components of bacterium were fractionated by differential centrifugation and salting out. Bacterial cell walls, cytoplasmic membrane fraction, soluble cytoplasmic proteins, and ribosomal fractions were isolated. Their effect on the mouse immune system was studied. Lymphocyte proliferation and immunoglobulin formation in vitro were stimulated by bacterial cell wall (BCW), cytoplasmic membrane (CMF), and ribosomal fractions. BCW and CMF increased antibody formation after intratracheal immunization of mice with influenza A and B viruses, and increased protection against subsequent infection with influenza virus. The BCW fraction even induced intersubtypic cross-protection: Mice immunized with A/California/7/04 (H3N2) + BCW were resistant to the infection by the highly pathogenic A/PR/8/34 (H1N1) virus.

Abbreviations

BAL

Bronchoalveolar lavage

BCW

Bacterial cell wall fraction

CMF

Cytoplasmic membrane fraction

ConA

Concavalin A

EID50

50% egg infective dose

FTS

Fetal calf serum

HT

Hemagglutination titer

iNOS

Inducible NO synthase

NAL

Nasal cavity lavage

PWM

Pokeweed mitogen

RiPF

Purified ribosomal fraction

RiCF

Crude ribosomal fraction

SCPF

Fraction of soluble cytoplasmic proteins

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2012