Folia Microbiologica

, 56:415

Molecular characterization of Staphylococcus pseudintermedius strains isolated from clinical samples of animal origin

  • D. Chrobak
  • M. Kizerwetter-Świda
  • M. Rzewuska
  • A. Moodley
  • L. Guardabassi
  • M. Binek
Article

DOI: 10.1007/s12223-011-0064-7

Cite this article as:
Chrobak, D., Kizerwetter-Świda, M., Rzewuska, M. et al. Folia Microbiol (2011) 56: 415. doi:10.1007/s12223-011-0064-7

Abstract

The aim of this study was to determine the species distribution among 44 randomly selected clinical isolates (30 mecA-positive and 14 mecA-negative) of animal origin previously identified as Staphylococcus intermedius by phenotypic tests and species-specific PCR amplification of the 16S rRNA gene. For this purpose, we used a multiplex PCR for the detection of the nuc gene and restriction fragment length polymorphism analysis of pta gene amplified by PCR. Both methods allow discrimination of Staphylococcus pseudintermedius from the other closely related members of the S. intermedius group and other coagulase-positive staphylococci isolated from animals. Genetic diversity of S. pseudintermedius strains was analyzed by staphylococcal protein A-encoding gene (spa) typing. Multiplex PCR method was used to identify staphylococcal cassette chromosome mec (SCCmec) type in mecA-positive strains. All isolates previously identified as S. intermedius were shown to belong to S. pseudintermedius. According to PCR-based SCCmec typing, SCCmecIII was the most prevalent type (n = 23), and solely seven isolates were designated as non-typeable. Furthermore, the assessment of spa-typing results revealed that the majority of all strains (n = 27) harbored spa type t02, and 17 strains were classified as non-typeable.

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2011

Authors and Affiliations

  • D. Chrobak
    • 1
  • M. Kizerwetter-Świda
    • 1
  • M. Rzewuska
    • 1
  • A. Moodley
    • 2
  • L. Guardabassi
    • 2
  • M. Binek
    • 1
  1. 1.Division of Bacteriology and Molecular Biology, Department of Preclinical Sciences, Faculty of Veterinary MedicineWarsaw University of Life SciencesWarsawPoland
  2. 2.Department of Veterinary Disease Biology, Faculty of Life SciencesUniversity of CopenhagenCopenhagenDenmark

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