Folia Microbiologica

, 56:23

Trk2 transporter is a relevant player in K+ supply and plasma-membrane potential control in Saccharomyces cerevisiae

Article

DOI: 10.1007/s12223-011-0009-1

Cite this article as:
Petrezsélyová, S., Ramos, J. & Sychrová, H. Folia Microbiol (2011) 56: 23. doi:10.1007/s12223-011-0009-1

Abstract

In Saccharomyces cerevisiae, TRK1 and TRK2 genes encode partially redundant K+ transporters. Direct involvement in K+ uptake has been shown for Trk1p since cells growing under limiting environmental K+ concentrations demand its presence. The biological role of Trk2p is less understood. In our experiments, TRK2 overexpression improved the ability of trk1 cells to grow in low K+ and led to a higher accumulation of K+. Using diS-C3(3) as a potentiometric probe, we revealed a higher hyperpolarization of trk2 cells compared to the wild type. In addition, the deletion of TRK2 in the trk1 genetic background increased the cell sensitivity to hygromycin B, spermine, and TMA. Our studies reinforced the conclusion that Trk1p is the prominent K+ uptake transporter and for the first time revealed that though Trk2p is much less effective, its activity contributes significantly to K+ supply and the maintenance of plasma-membrane potential.

Abbreviations

∆ψ

Plasma membrane potential

TMA

Tetramethylammonium

diS-C3(3)

3,3′-dipropylthiacarbocyanine iodide

SD

Synthetic minimal medium

LB

Luria–Bertani (medium)

YPD

Rich medium

Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2011

Authors and Affiliations

  1. 1.Department of Membrane TransportInstitute of Physiology, Academy of Sciences of the Czech Republic, v.v.i.Prague 4Czech Republic
  2. 2.Department of Microbiology, ETSIAM, Edificio Severo Ochoa, Campus de RabanalesUniversity of CórdobaCórdobaSpain