Folia Microbiologica

, Volume 55, Issue 5, pp 489–496

Bacterial community composition of the gut microbiota of Cylindroiulus fulviceps (diplopoda) as revealed by molecular fingerprinting and cloning

  • B. A. Knapp
  • J. Seeber
  • A. Rief
  • E. Meyer
  • H. Insam
Article

DOI: 10.1007/s12223-010-0081-y

Cite this article as:
Knapp, B.A., Seeber, J., Rief, A. et al. Folia Microbiol (2010) 55: 489. doi:10.1007/s12223-010-0081-y

Abstract

Bacterial clone libraries of the gut microbiota of nurtured and starved Cylindroiulus fulviceps specimens displayed the predominance of the phyla Bacteroidetes (55 and 37 %, respectively) and Proteobacteria (40 and 35 %, respectively) and a high similarity to bacteria previously detected in the intestinal tract of termites and beetles, which are known to harbor symbiotic bacteria essential for digestive activity. Bacterial isolates were dominated by Proteobacteria (74 %), followed by members of the phyla Actinobacteria, Firmicutes and Bacteroidetes. PCR-DGGE fingerprints of the gut samples showed that intestinal bacteria were affected by starvation, although the change was not significant.

Abbreviations

DGGE

denaturing gradient gel electrophoresis

ICD

intestines of diplopods fed with cow dung

ISA

intestines of starved diplopods

OTUs

operational taxonomic units

PCO

principal coordinate analysis

RDP

ribosomal database project

Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2010

Authors and Affiliations

  • B. A. Knapp
    • 1
  • J. Seeber
    • 2
  • A. Rief
    • 2
  • E. Meyer
    • 2
  • H. Insam
    • 1
  1. 1.Institute of MicrobiologyUniversity of InnsbruckInnsbruckAustria
  2. 2.Institute of EcologyUniversity of InnsbruckInnsbruckAustria