Folia Microbiologica

, Volume 54, Issue 2, pp 111–114

Phytase activity in rabbit cecal bacteria

  • M. Marounek
  • N. Břeňová
  • O. Suchorská
  • J. Mrázek
Article

DOI: 10.1007/s12223-009-0016-7

Cite this article as:
Marounek, M., Břeňová, N., Suchorská, O. et al. Folia Microbiol (2009) 54: 111. doi:10.1007/s12223-009-0016-7

Abstract

The presence of phytase activity was demonstrated in 26 strains of rabbit cecal bacteria. In 25 strains a low phytase activity, 0.10–0.62 μmol phosphate released per min per mg protein, was found. High activity (2.61 μmol/min per mg protein) was found in the strain PP2 identified as Enterococcus hirae. Phytase activity was cell-associated, being higher in the cell extract than in the cell walls. Extracellular phytase activity and cell-associated phosphatase activity were not detected. Phytase activity was optimal around pH 5.0, which is below the physiological cecal pH range. The Km determined using the Lineweaver-Burk plot was 0.19 μmol/mL. Cations Fe3+, Cu2+ and Zn2+ at 0.5 mmol/L decreased phytase activity in sonicated cells of E. hirae by 99.4, 90.7 and 96.5 %, respectively. In contrast, Mg2+ increased activity by 11.0 %. Characteristics of E. hirae phytase (pH optimum, Km, cation sensitivity) were similar to those of other bacterial phytases reported in the literature. Other bacteria with a high phytase activity may be present in the rabbit cecum but remain to be identified.

Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2009

Authors and Affiliations

  • M. Marounek
    • 1
    • 2
  • N. Břeňová
    • 1
  • O. Suchorská
    • 1
  • J. Mrázek
    • 1
  1. 1.Institute of Animal Physiology and GeneticsAcademy of Sciences of the Czech RepublicPragueCzech Republic
  2. 2.Institute of Animal SciencePragueCzech Republic
  3. 3.Institute of BiotechnologyLviv National Academy of Veterinary MedicineLvovUkraine