Papers

Folia Microbiologica

, Volume 53, Issue 5, pp 395-401

First online:

Sequence analysis and heterologous expression of the lincomycin biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC 25466

  • M. KoběrskáAffiliated withInstitute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i. Email author 
  • , J. KopeckýAffiliated withInstitute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • , J. OlšovskáAffiliated withInstitute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • , M. JelínkováAffiliated withInstitute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • , D. UlanovaAffiliated withInstitute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • , P. ManAffiliated withInstitute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • , M. FliegerAffiliated withInstitute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • , J. JanataAffiliated withInstitute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

A cosmid bearing an insert of 38 217 bp covering the gene cluster and its flanking regions of type strain Streptomyces lincolnensis ATCC 25466 was sequenced. Two relatively extensive sequence changes and several hundred point mutations were identified if compared with the previously published sequence of the lincomycin (Lin) industrial strain S. lincolnensis 78-11. Analysis of the cluster-flanking regions revealed its localization within the genome of the ATCC 25466 strain. The cluster-bearing cosmid was integrated into the chromosome of Lin non-producing strains S. coelicolor CH 999 and S. coelicolor M 145. The modified strains heterologously produced Lin but the level dropped to ≈1–3 % of the production in the ATCC 25466 strain.