Folia Microbiologica

, Volume 53, Issue 5, pp 395–401

Sequence analysis and heterologous expression of the lincomycin biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC 25466

Authors

    • Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • J. Kopecký
    • Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • J. Olšovská
    • Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • M. Jelínková
    • Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • D. Ulanova
    • Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • P. Man
    • Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • M. Flieger
    • Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
  • J. Janata
    • Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i.
Papers

DOI: 10.1007/s12223-008-0060-8

Cite this article as:
Koběrská, M., Kopecký, J., Olšovská, J. et al. Folia Microbiol (2008) 53: 395. doi:10.1007/s12223-008-0060-8

Abstract

A cosmid bearing an insert of 38 217 bp covering the gene cluster and its flanking regions of type strain Streptomyces lincolnensis ATCC 25466 was sequenced. Two relatively extensive sequence changes and several hundred point mutations were identified if compared with the previously published sequence of the lincomycin (Lin) industrial strain S. lincolnensis 78-11. Analysis of the cluster-flanking regions revealed its localization within the genome of the ATCC 25466 strain. The cluster-bearing cosmid was integrated into the chromosome of Lin non-producing strains S. coelicolor CH 999 and S. coelicolor M 145. The modified strains heterologously produced Lin but the level dropped to ≈1–3 % of the production in the ATCC 25466 strain.

Abbreviations

ABC

ATP-binding cassette

Mtl

methylthiolincosamide

l-Dop

3,4-dihydroxy-L-phenylalanine

Ndl

N-demethyllincomycin

int

integrase gene of phage φC31

NDLS

N-demethyllincomycin synthetase

Lin

lincomycin

PCR

polymerase chain reaction

lmb

Lin biosynthetic genes

Ppl

4-propyl-L-proline

MS

mass spectrometry

UPLC

ultra performance liquid chromatography

Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2008