Microgravity Science and Technology

, Volume 21, Issue 3, pp 217–223

Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity Conditions

  • Joke De Gelder
  • Peter Vandenabeele
  • Patrick De Boever
  • Max Mergeay
  • Luc Moens
  • Paul De Vos
Original Article

DOI: 10.1007/s12217-008-9037-0

Cite this article as:
De Gelder, J., Vandenabeele, P., De Boever, P. et al. Microgravity Sci. Technol (2009) 21: 217. doi:10.1007/s12217-008-9037-0

Abstract

In this study, the effect of low-shear microgravity on the metabolism of Cupriavidus metallidurans LMG 1195 was studied with Raman spectroscopy. Therefore, the strain was cultured for 24 or 48 h in a rotating wall vessel to simulate microgravity (SMG) and in a control setup. The differences in Raman spectra recorded from both setups after 24 h of culturing were small. The most prominent features in a difference spectrum, calculated between the mean spectra from the microgravity and the control setup separately, could be assigned to the presence of poly-β-hydroxybutyrate (PHB). SMG seems to yield a higher PHB production after 24 h of culturing. Additional processing of the spectra suggested that SMG induced also other changes in the carbon-metabolism. After 48 h, similar results were found for the carbon metabolism, while PHB concentrations were reduced in SMG compared to the control. However, these differences could also be caused by interfering effects that may occur in the bioreactors after a prolonged incubation time.

Keywords

Raman spectroscopyMicrogravityBacteriaCupriavidus metalliduransPHBMetabolomics

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Joke De Gelder
    • 1
  • Peter Vandenabeele
    • 2
  • Patrick De Boever
    • 3
    • 5
  • Max Mergeay
    • 3
  • Luc Moens
    • 1
  • Paul De Vos
    • 4
  1. 1.Department of Analytical ChemistryGhent UniversityGhentBelgium
  2. 2.Department of Archeology and Ancient History of EuropeGhent UniversityGhentBelgium
  3. 3.Laboratory for Molecular and Cellular BiologyInstitute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK•CEN)MolBelgium
  4. 4.Laboratory of Microbiology (Lm-Ugent)Ghent UniversityGhentBelgium
  5. 5.Environmental ToxicologyFlemish Institute for Technological ResearchGeelBelgium