Research Paper

Journal of Micro-Nano Mechatronics

, Volume 7, Issue 4, pp 131-136

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Cell palpation with an optically trapped particle

  • Tadao SugiuraAffiliated withGraduate School of Information Science, Nara Institute of Science and Technology Email author 
  • , Hideaki MiyoshiAffiliated withGraduate School of Information Science, Nara Institute of Science and Technology
  • , Tetsu NishioAffiliated withGraduate School of Information Science, Nara Institute of Science and Technology
  • , Ayae HondaAffiliated withFaculty of Bioscience and Applied Chemistry, Housei University

Abstract

We have developed a cell palpation system to investigate cell stiffness from the reaction force generated on a particle that is fixed on a cell. In this method, a particle is used as a probe, and is manipulated towards a cell using optical tweezers. Using this method, we can obtain information of local stiffness of a cell. We investigate focal adhesion formation of a cell probed by a particle and we report different particle coating utilized for attaching certain protein in cell membrane. Also we discuss the effects of endocytosis.

Keywords

Optical tweezers Cell stiffness Micromanipulation