Journal of Shanghai Jiaotong University (Science)

, Volume 15, Issue 5, pp 637–640

Construction of eukaryotic recombinant vector of renalase and its expression as a eukaryotic protein

  • Feng Wang王 锋
  • Nian-song Wang汪年松
  • Tao Xing形 涛
Article

DOI: 10.1007/s12204-010-1061-8

Cite this article as:
Wang, F., Wang, N. & Xing, T. J. Shanghai Jiaotong Univ. (Sci.) (2010) 15: 637. doi:10.1007/s12204-010-1061-8

Abstract

Renalase is a secreted amine oxidase that metabolizes catecholamines. It has been proposed to modulate blood pressure and heart rate and its downregulation might result in hypertension. Despite its potential relevance for human health, the biochemical characterization of renalase is still scarce. The aim of this study is to synthesize the human renalase eukaryotic protein by genetic engineering. The human renalase gene was amplified by polymerase chian reaction (PCR). After digestion by BamH I and Xho I enzymes, the DNA fragments were cloned into the transfer vector, pFastBacHTb-Fc, to generate the pFastBacHTb-renalase expression vector. The ligation products were transformed into E. coli DH10Bac to obtain recombinant transposon rBacmid-renalase. The recombinant transposon was further transferred into insect high-V cells, and the recombinant human renalase eukaryotic protein was expressed successfully.

Key words

renalaseexpressioneukaryon

CLC number

R 692.5

Copyright information

© Shanghai Jiaotong University and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Feng Wang王 锋
    • 1
  • Nian-song Wang汪年松
    • 1
  • Tao Xing形 涛
    • 1
    • 2
  1. 1.Department of Nephrologythe Sixth People’s Hospital Affiliated to Shanghai Jiaotong UniversityShanghaiChina
  2. 2.Australian School of Advanced MedicineMacquarie UniversitySydneyAustralia