Article

Journal of Shanghai Jiaotong University (Science)

, Volume 15, Issue 5, pp 637-640

First online:

Construction of eukaryotic recombinant vector of renalase and its expression as a eukaryotic protein

  • Feng Wang王 锋Affiliated withDepartment of Nephrology, the Sixth People’s Hospital Affiliated to Shanghai Jiaotong University
  • , Nian-song Wang汪年松Affiliated withDepartment of Nephrology, the Sixth People’s Hospital Affiliated to Shanghai Jiaotong University Email author 
  • , Tao Xing形 涛Affiliated withDepartment of Nephrology, the Sixth People’s Hospital Affiliated to Shanghai Jiaotong UniversityAustralian School of Advanced Medicine, Macquarie University

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Abstract

Renalase is a secreted amine oxidase that metabolizes catecholamines. It has been proposed to modulate blood pressure and heart rate and its downregulation might result in hypertension. Despite its potential relevance for human health, the biochemical characterization of renalase is still scarce. The aim of this study is to synthesize the human renalase eukaryotic protein by genetic engineering. The human renalase gene was amplified by polymerase chian reaction (PCR). After digestion by BamH I and Xho I enzymes, the DNA fragments were cloned into the transfer vector, pFastBacHTb-Fc, to generate the pFastBacHTb-renalase expression vector. The ligation products were transformed into E. coli DH10Bac to obtain recombinant transposon rBacmid-renalase. The recombinant transposon was further transferred into insect high-V cells, and the recombinant human renalase eukaryotic protein was expressed successfully.

Key words

renalase expression eukaryon

CLC number

R 692.5