The cloning and expression of renalase and the preparation of its monoclonal antibody

  • Feng Wang (王 锋)
  • Nian-song Wang (汪年松)
  • Tao Xing (邢 涛)
  • Yang Cao (曹 阳)
  • Hai-yan Xiang (向海燕)
Article

DOI: 10.1007/s12204-009-0376-9

Cite this article as:
Wang, F., Wang, N., Xing, T. et al. J. Shanghai Jiaotong Univ. (Sci.) (2009) 14: 376. doi:10.1007/s12204-009-0376-9

Abstract

To obtain the recombination protein of renalase and prepare the monoclonal antibody, the human renalase gene was amplified from human kidney by specific primer and cloned the DNA fragments into the pET22b. After verification of the positive clones, the gene was transformed to E. coli BL21 to express the protein with 6His on C terminal. The Balb/c mouse was immunized with the purified protein to prepare the monoclonal antibody by hybidoma technique. The renalase protein was reconstructed and 2 strains of the hybidoma which can stable secrete renalase were obtained. The monoclonal antibody can both react with the both recombinant and human serum renalase.

Key words

renalase expression monoclonal antibody 

CLC number

R 692.5 

Copyright information

© Shanghai Jiaotong University and Springer-Verlag GmbH 2009

Authors and Affiliations

  • Feng Wang (王 锋)
    • 1
  • Nian-song Wang (汪年松)
    • 1
  • Tao Xing (邢 涛)
    • 2
  • Yang Cao (曹 阳)
    • 3
  • Hai-yan Xiang (向海燕)
    • 1
  1. 1.Department of Nephrologythe 6th People’s Hospital Affiliated to Shanghai Jiaotong UniversityShanghaiChina
  2. 2.the Australian School of Advanced MedicineMacquarie UniversitySydneyAustralia
  3. 3.School of Life Science & BiotechnologyShanghai Jiaotong UniversityShanghaiChina

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