Cell Stress and Chaperones

, Volume 19, Issue 2, pp 263–270

Exercise reduces cellular stress related to skeletal muscle insulin resistance

  • Mariana Aguiar de Matos
  • Vinícius de Oliveira Ottone
  • Tamiris Campos Duarte
  • Pâmela Fiche da Matta Sampaio
  • Karine Beatriz Costa
  • Cheyenne Alves Fonseca
  • Miguel Pontes Correa Neves
  • Suzanne Maria Schneider
  • Pope Moseley
  • Cândido Celso Coimbra
  • Flávio de Castro Magalhães
  • Etel Rocha-Vieira
  • Fabiano Trigueiro Amorim
Original Paper

DOI: 10.1007/s12192-013-0453-8

Cite this article as:
Matos, M.A., Ottone, V.O., Duarte, T.C. et al. Cell Stress and Chaperones (2014) 19: 263. doi:10.1007/s12192-013-0453-8

Abstract

This study sought to evaluate the effects of a single session of exercise on the expression of Hsp70, of c-jun N-terminal kinase (JNK), and insulin receptor substrate 1 serine 612 (IRSser612) phosphorylation in the skeletal muscle of obese and obese insulin-resistant patients. Twenty-seven volunteers were divided into three experimental groups (eutrophic insulin-sensitive, obese insulin-sensitive, and obese insulin-resistant) according to their body mass index and the presence of insulin resistance. The volunteers performed 60 min of aerobic exercise on a cycle ergometer at 60 % of peak oxygen consumption. M. vastus lateralis samples were obtained before and after exercise. The protein expressions were evaluated by Western blot. Our findings show that compared with paired eutrophic controls, obese subjects have higher basal levels of p-JNK (100 ± 23 % vs. 227 ± 67 %, p = 0.03) and p-IRS-1ser612 (100 ± 23 % vs. 340 ± 67 %, p < 0.001) and reduced HSP70 (100 ± 16 % vs. 63 ± 12 %, p < 0.001). The presence of insulin resistance results in a further increase in p-JNK (460 ± 107 %, p < 0.001) and a decrease in Hsp70 (46 ± 5 %, p = 0.006), but p-IRS-1ser612 levels did not differ from obese subjects (312 ± 73 %, p > 0.05). Exercise reduced p-JNK in obese insulin-resistant subjects (328 ± 33 %, p = 0.001), but not in controls or obese subjects. Furthermore, exercise reduced p-IRS-1ser612 for both obese (122 ± 44 %) and obese insulin-resistant (185 ± 36 %) subjects. A main effect of exercise was observed in HSP70 (p = 0.007). We demonstrated that a single session of exercise promotes changes that characterize a reduction in cellular stress that may contribute to exercise-induced increase in insulin sensitivity.

Keywords

ExerciseInsulin resistanceObesityHeat shock proteinCellular stress

Copyright information

© Cell Stress Society International 2013

Authors and Affiliations

  • Mariana Aguiar de Matos
    • 1
  • Vinícius de Oliveira Ottone
    • 1
  • Tamiris Campos Duarte
    • 1
  • Pâmela Fiche da Matta Sampaio
    • 1
  • Karine Beatriz Costa
    • 1
  • Cheyenne Alves Fonseca
    • 1
  • Miguel Pontes Correa Neves
    • 1
  • Suzanne Maria Schneider
    • 2
  • Pope Moseley
    • 2
  • Cândido Celso Coimbra
    • 3
  • Flávio de Castro Magalhães
    • 1
  • Etel Rocha-Vieira
    • 1
  • Fabiano Trigueiro Amorim
    • 1
  1. 1.Exercise Physiology Laboratory, Healthy and Biological Sciences FacultyFederal University of Jequitinhonha and Mucuri ValleysDiamantinaBrazil
  2. 2.University of New MexicoAlbuquerqueUSA
  3. 3.Endocrinology Laboratory, Biological Sciences InstituteFederal University of Minas GeraisBelo HorizonteBrazil